Xiao Song, Peng Zhao, Xi Duan, Chang-li Wang, Han-shuang Deng
{"title":"[Study on Deproteinization of Polysaccharides from Dioscorea nipponica].","authors":"Xiao Song, Peng Zhao, Xi Duan, Chang-li Wang, Han-shuang Deng","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides.</p><p><strong>Methods: </strong>The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated.</p><p><strong>Results: </strong>PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method.</p><p><strong>Conclusion: </strong>The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides.
Methods: The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated.
Results: PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method.
Conclusion: The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
分享
京公网安备 11010802042870号
京ICP备2023020795号-1
求助内容：
应助结果提醒方式：
扫码关注我们
