Cloning, localization and bioinformatics analysis of a gene encoding an odorant-binding protein (OBP) in Anoplophora glabripennis (Motschulsky).

Q4 Neuroscience Invertebrate Neuroscience Pub Date : 2018-08-31 DOI:10.1007/s10158-018-0215-5
Yu Li, Huien Li, Zhigang Wang, Danyang Gao, Kun Xiao, Aihua Yan
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引用次数: 2

Abstract

Anoplophora glabripennis (Motschulsky) has an advanced and complicated olfactory system to identify hosts, mates and spawning locations, and odorant-binding proteins (OBPs) play a key role by binding to volatile materials from different hosts. The full-length cDNA sequence of an OBP, AglaOBP, was cloned by RACE from an antenna cDNA library, and the protein structure and function were predicted by bioinformatics analysis. Gene temporal and spatial expression was detected by real-time qPCR. AglaOBP had distinctive sequence, location and expression profiles compared with other OBPs of A. glabripennis, as it was found in different tissues, and the highest expression was in the elytrums. The possible physiological functions of this OBP were discussed. These findings help elucidate the physiology of this pest and provide a new potential target for pest control.

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光肩天牛气味结合蛋白(OBP)基因的克隆、定位及生物信息学分析
光肩天牛(Anoplophora glabripennis, Motschulsky)具有先进而复杂的嗅觉系统来识别寄主、配偶和产卵地点,气味结合蛋白(OBPs)通过结合不同寄主的挥发性物质发挥关键作用。利用RACE技术从天线cDNA文库中克隆出OBP AglaOBP的全长cDNA序列,并通过生物信息学分析对其结构和功能进行了预测。采用实时荧光定量pcr检测基因的时空表达。由于AglaOBP存在于不同的组织中,且在鞘翅中表达量最高,因此与光肩棘猴其他obp相比,其序列、位置和表达谱都有明显的差异。并对其可能的生理功能进行了讨论。这些发现有助于阐明该害虫的生理特性,并为害虫防治提供新的潜在靶点。
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Invertebrate Neuroscience
Invertebrate Neuroscience NEUROSCIENCES-
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期刊介绍: Invertebrate Neurosciences publishes peer-reviewed original articles, reviews and technical reports describing recent advances in the field of invertebrate neuroscience. The journal reports on research that exploits the simplicity and experimental tractability of the invertebrate preparations to underpin fundamental advances in neuroscience. Articles published in Invertebrate Neurosciences serve to highlight properties of signalling in the invertebrate nervous system that may be exploited in the field of antiparisitics, molluscicides and insecticides. Aspects of particular interest include: Functional analysis of the invertebrate nervous system; Molecular neuropharmacology and toxicology; Neurogenetics and genomics; Functional anatomy; Neurodevelopment; Neuronal networks; Molecular and cellular mechanisms of behavior and behavioural plasticity.
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