[Chemical Constituents from A Taditional Mongolian Medicine Clematis aethusifolia].

中药材 Pub Date : 2016-07-01

Yan-xi Chang, Bao-quan Bao, Xuan Zhang, J I Rimubatu, Ping Zhang

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Abstract

Objective: To investigate the chemical constituents of Clematis aethusifolia,a traditional Mongolian medicine for resolving hard lump.

Methods: Bioactive guided isolation and purification of Clematis aethusifolia was performed by normal and reverse phase column chromatography, Sephadex LH-20 and preparation HPLC. The structures were identified by 1D,2D-NMR and MS spectral analysis and comparison with literature data. Cytotoxicity of compounds were determined by CCK-8 cell staining method for detecting growth inhibition to five kinds of human solid tumor cell lines.

Results: Eight compounds were isolated and identified as vanillicacid( 1),protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity.

Conclusion: All the compounds are isolated from this plant for the first time, Results: Eight compounds were isolated and identified as vanillicacid( 1), protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. compounds 3,6 ~ 8 are isolated from Clematis genus for the first time, compounds 6 and 8 are isolated from Ranunculaceae family for the first time. Compounds 2,8 may be the effective components in Clematis aethusifolia.

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蒙药铁线莲的化学成分。

目的:研究治疗硬疙瘩的蒙药铁线莲的化学成分。方法:采用正、反相柱层析、Sephadex LH-20和制备高效液相色谱法对铁线莲进行生物活性引导分离纯化。通过一维、二维核磁共振和质谱分析，并与文献数据进行对比，对其结构进行了鉴定。采用CCK-8细胞染色法检测化合物对5种人实体瘤细胞系的生长抑制作用。结果:共分离得到8个化合物，分别为香草酸(1)、原儿茶酸(2)、(+)-松脂醇二葡糖苷(3)、(+)-松脂醇-4′-O-β- d -葡萄糖吡喃苷(4)、(+)-丁香精醇-4′-O-β-葡萄糖吡喃苷(5)、7,9,9′-三羟基-3,3′-二甲氧基-8-O-4′-新木质素-4-O-β- d -葡萄糖吡喃苷(6)、丁基-β- d -果糖吡喃苷(7)、丁基-β- d -果糖吡喃苷(8)，化合物2,8表现出较强的细胞毒活性。结果:从该植物中分离得到8个化合物，分别为香草酸(1)、原儿茶酸(2)、(+)-松脂醇二葡糖苷(3)、(+)-松脂醇-4′-O-β- d -葡萄糖吡喃苷(4)、(+)-丁香精醇-4′-O-β-葡萄糖吡喃苷(5)、7,9,9′-三羟基-3,3′-二甲氧基-8-O-4′-新木酚-4-O-β- d -葡萄糖吡喃苷(6)、丁基-β- d -果糖吡喃苷(7)、丁基-β- d -果糖吡喃苷(8)，其中化合物2,8表现出较强的细胞毒活性。其中化合物3、6 ~ 8为首次从铁线莲属植物中分离得到，化合物6、8为首次从毛茛科植物中分离得到。化合物2、8可能是铁线莲的有效成分。

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中药材

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11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.