Yu-ling Pan, Qi Zhao, Zhen-qiao Song, Jian-hua Wang
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引用次数: 0
Abstract
Objective: On the basis of previous studies, to construct a genetic map of Salvia miltiorrhiza by using EST-SSR primers, to build a platform for positioning important traits relating to genes, the cloning and molecular marker-assisted in breeding of new varieties of Salvia miltiorrhiza.
Methods: A total of 411 EST-SSR primers were used for PCR amplification to screen polymorphic markers in F1 mapping population derived from a cross between Salvia miltiorrhiza, two parents of which were the cultivars of ZH74 and BH18. Combined with the molecular marker data of previous studies, Joinmap 4. 0 software was used for map integration.
Results: 411EST-SSR primers were screened from two parents, a total of 328 pairs of primers were amplified stable products,164 pairs of polymorphic primers were obtained.
Conclusion: A linkage map of Salvia miltiorrhiza with 150 marker high density genetic is constructed.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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