[The Effect in Extraction of Different Ratio of Dahuang-Taoren for Ten Kinds of Chemical Constituents from Rhizoma Rhei].

中药材 Pub Date : 2016-07-01

Yong-gang Yan, Li-min Yin, Hong-yan Wang, Jie Wang, Jiao-jiao Liu, Chong Deng

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Abstract

Objective: To investigate the effect of Dahuang-Taoren with different proportion of extraction amount changes of ten kinds of chemical constituents in Rhizoma Rhei.

Methods: Uniform method to set different ratio( 1∶ 5,2∶ 5,2∶ 3,1∶ 1,3∶ 2,5∶ 2,5∶ 1),and set the control group ( Dahuang-Taoren( 1 ∶ 0). HPLC was used to determine the content of ten constituents as gallic acid,( +)-catechin,sennoside B,anthraquinones( aloe-emodin,rhein,emodin,chrysophanol,physcion,chrysophanol-1-O-glucoside,emodin major grape glycoside) in different ratio of drug. Different proportions of Dahuang-Taoren on the extraction amount of ten chemical components in Rhizoma Rhei changes were analyzed. .

Results: Compared to the control group, Dahuang-Taoren with different ratio( 5∶ 1,5∶ 2,3∶ 2) in a sample with increasing proportion of Taoren,the extraction amount of ten kinds of constituents of Rhizoma Rhei gradually decreased;Dahuang-Taoren with ratio of 1∶ 1,ten kinds of constituents in extraction of total amount arrived minimum value. Dahuang-Taoren with different ratio( 2∶ 3,2∶ 5,1∶ 5) in a sample with increasing proportion of Taoren,the extraction amount of gallic acid,( +) catechin,chrysophanol of Rhizoma Rhei increased significantly.

Conclusion: There is obvious change in chemical constituents of the extraction amount of Rhizoma Rhei with the change of the ratio in Dahuang-Taoren, and Dahuang-Taoren with the ratio( 2∶ 3,2∶ 5,1∶ 5),the extraction amount of gallic acid,( +)-catechin,sennoside B,aloe-emodin,emodin,chrysophanol,physcion,chrysophanol-1-O-glucoside are significantly higher than control group.

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[大黄-桃仁不同配比对大黄10种化学成分提取的影响]。

目的:考察大黄桃仁在不同比例下对大黄桃仁的影响，探讨大黄中10种化学成分的提取量变化。方法:采用统一的方法设置不同比例(1∶5、2∶5、2∶3、1∶1、3∶2、5∶2、5∶1)，并设置对照组(大黄-桃仁(1∶0))，采用高效液相色谱法测定不同比例药物中没食子酸、(+)-儿茶素、senno苷B、蒽醌类(芦荟大黄素、大黄素、大黄酚、大黄酚-1- o -葡萄糖苷、大黄素主要葡萄糖苷)的含量。结果:与对照组相比，不同配比(5∶1、5∶2、3∶2)的大黄桃仁对大黄中10种化学成分的提取量随着大黄桃仁配比的增加，大黄中10种化学成分的提取量逐渐减少;大黄桃仁配比为1∶1时，大黄中10种化学成分的提取总量达到最小值。大黄-桃仁不同比例(2∶3、2∶5、1∶5)的样品中，随着桃仁比例的增加，大黄中没食子酸、(+)儿茶素、大黄酚的提取量显著增加。结论:大黄桃仁及大黄桃仁比例(2∶3、2∶5、1∶5)下大黄化学成分的提取量随比例的变化有明显变化，未食子酸、(+)-儿茶素、senno苷B、芦荟大黄素、大黄素、大黄酚、大黄酚-1- o -葡萄糖苷的提取量显著高于对照组。

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.