[Preparation of Salviae Miltiorrhiza and Ligustrazine Hydrochloride Nasal Thermosensitive in Situ Gel and Study on Characterization of Its Nasal Mucosal Permeability].

Abstract

Objective: To prepare the salviae miltiorrhiza and ligustrazine hydrochloride nasal thermosensitive in situ gel and to study the characterization of its nasal mucosal permeability.

Methods: With gelling temperature as the dependent variable, the contents of poloxamer 407( P407), poloxamer 188( P188) and PEG-6000 as independent variables. The best prescription was optimized by central composite design-response surface methodology. The release in vitro and the skin permeation ability were evaluated in Franz diffusion cell.

Results: The optimal formulation composed with the dosage of P407,P188 and PEG-6000 were 18%,7% and 1%,respectively. The cumulative release in vitro was over 70% after 12 h, and the release curve conformed to the first-order kinetic equation. The cumulative permeation of salviae miltiorrhiza and ligustrazine hydrochloride in the gel were 2 649. 77 μg / cm2 and 119. 72 μg / cm2 after 12h.

Conclusion: The central composite design-response surface methodolog was stable and feasible for preparation of nasal thermosensitive in situ gel of salviae miltiorrhiza and ligustrazine hydrochloride, the drug-loaded gel has sustained release effect.