[Study on Total Triterpenoids Content of Enrichment Mineral Elements of Inonotus Obliquus].

Abstract

Objective: To study the influence of different mineral elements enrichment of calcium, iron, and zinc on the growth of the mycelium from Inonotus obliquus, to explore the influence on total triterpenoids content of Inonotus obliquus.

Methods: Mineral elements were enriched on Inonotus obliquus, total triterpenoids extraction methods and determination methods were optimized, and total triterpenoids content of Inonotus obliquus were compared through different mineral elements enrichment.

Results: The mycelial growth and yield were promoted obviously when 1 000 mg/L Ca(NO3)2 was added in the culture medium, and the calcium enrichment rate could reach to 0.07%. The most appropriate iron source was Fe2(SO4)3, and the iron enrichment rate could reach to 0.29% when 300 mg/L Fe2(SO4)3 was added. 175 mg/L Zn SO4 led to the strongest mycelium growth vigor, and the zinc enrichment rate could reach to 0.57%. The total triterpenoids content of Inonotus obliquus was the highest (0.058 mg/mL) as enrichment of calcium element, the total triterpenoids content of Inonotus obliquus was the least (0.04 mg/mL) as enrichment of iron elements.

Conclusion: The total triterpenoids content of Inonotus obliquus was the highest when calcium element was enriched. This study provides a new method and basic data for the factory production of Inonotus obliquus.