[Tissue Distribution of Alkaloids from Coptidis Rhizoma in Rats Determined by UPLC-MS].

中药材 Pub Date : 2016-08-01

Xiao-lin Bi, Ya Wang, Yu-mei Chi, Liu-qing Di, Jun-song Li

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引用次数: 0

Abstract

Objective: To develop an UPLC-MS method for simultaneous determination of alkaloids from Coptidis Rhizoma in rat tissues, and to study the tissue distribution of alkaloids from Coptidis Rhizoma in rats.

Methods: The samples were extracted with ethyl acetate, and analyzed by UPLC-MS with acetonitrile-0. 2% formic acid solution in a gradient elution mobile phase, the flow rate was 0. 2m L/min. Tetrahydropalmatine was used as an internal standard. The mass spectrometer was operated in selected reaction monitoring( SIM) mode with positive electrospray ionization, the transition were m/z 191. 904 /118. 973( noroxyhydrastinine), m/z 335. 877 /308. 072( 8-ocoptisine),m/z 351. 94 /294. 554( palmatine chloride),m/z 335. 94 /262. 112( epiberberine), m/z 337. 94 /322. 422( columbamine), m/z 319. 904 /292. 037( coptisine), m/z 355. 977 /192. 036( tetrahydropalmatine),m/z 335. 94 /320. 036( berberine hydrochloride),m/z 351. 94 /321. 995( oxyberberin), m/z 337. 94 /322. 949( jatrorrhizine respectively).

Results: Excellent linearity was observed in all alkaloids in their linear range( r & 0. 9901). The RSD of precision of the developed method was less than 15%,and the accuracy and stability were less than ± 15%,the extraction recovery was 72. 1% ~ 82. 9% with RSD less than 15%. Coptisine,epiberberine,berberine,jatrorrhizine,columbamine,palmatine were widely distributed in rat tissues. Noroxyhydrastinine,8-ocoptisine,oxyberberin could only be determined in liver and heart or kidney.

Conclusion: The established method is simple and accurate. Satisfactory results are obtained with applying this method to the tissue distribution study of alkaloids from Coptidis Rhizoma.

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[超高效液相色谱-质谱法测定黄连生物碱在大鼠体内的组织分布]。

目的:建立同时测定大鼠组织中黄连生物碱含量的UPLC-MS方法，研究黄连生物碱在大鼠体内的组织分布。方法:采用乙酸乙酯提取，乙腈-0超高效液相色谱-质谱分析。2%甲酸溶液，梯度洗脱流动相，流速为0。2 m L / min。四氢巴马汀作为内标。质谱计工作在选择性反应监测(SIM)模式下，电喷雾电离，跃迁为m/z 191。904/118。973(去氧肼)，m/z 335。877/308。072(8-ocoptisine)，m/z 351。94/294。554(palmatine chloride)，m/z 335。94/262。112(小檗碱)，m/z 337。94/322。422(钶钽铁矿)，m/z 319。904/292。037(coptisine)， m/z 355。977/192。036(四氢巴马汀)，m/z 335。94/320。036(盐酸小檗碱)，m/z 351。94/321。995(oxyberberin)， m/ z337。94/322。949(分别为黄麻根碱)。结果:各生物碱在线性范围(r & 0)内呈良好的线性关系。9901)。该方法精密度的RSD < 15%，准确度和稳定性<±15%，提取回收率为72。1% ~ 82。9%， RSD小于15%。黄连碱、小檗碱、小檗碱、黄根碱、柱状碱、棕榈碱广泛分布于大鼠组织中。去甲氧肼、8-奥古斯汀、oxyberberin只能在肝脏、心脏或肾脏中检测到。结论:所建立的方法简便、准确。将该方法应用于黄连生物碱的组织分布研究，取得了满意的结果。

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来源期刊

中药材

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.