{"title":"[SCoT Analysis on Genetic Diversity of Medicinal Fungus Cordyceps militaris].","authors":"Ting Li, Bin Song, Min Li","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To analyze the genetic diversity of medicinal fungus of 27 strains of Cordyceps militaris.</p><p><strong>Methods: </strong>Unweighted pair-group method with arithmetic means( UPGMA) and principal coordinate analysis( PCoA) were used to determine the genetic diversity of 27 Cordyceps militaris strains based on start codon targeted polymorphism( SCoT).</p><p><strong>Results: </strong>Based on eight informative primers,a total of 59 bands were produced,and 94. 33% of them were polymorphic. The average of polymorphism bands was 6. 875. The results suggested that great genetic diversity existed in Cordyceps militaris. Basically,27 strains were separated into six groups using UPGMA method,while three groups when using PCoA method,which showed that genetic diversity of Cordyceps militaris samples were related to their geographical origins.</p><p><strong>Conclusion: </strong>SCoT markers are informative and could be used to detect polymorphism for medicinal fungus of Cordyceps militaris strains.</p>","PeriodicalId":15312,"journal":{"name":"中药材","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中药材","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To analyze the genetic diversity of medicinal fungus of 27 strains of Cordyceps militaris.
Methods: Unweighted pair-group method with arithmetic means( UPGMA) and principal coordinate analysis( PCoA) were used to determine the genetic diversity of 27 Cordyceps militaris strains based on start codon targeted polymorphism( SCoT).
Results: Based on eight informative primers,a total of 59 bands were produced,and 94. 33% of them were polymorphic. The average of polymorphism bands was 6. 875. The results suggested that great genetic diversity existed in Cordyceps militaris. Basically,27 strains were separated into six groups using UPGMA method,while three groups when using PCoA method,which showed that genetic diversity of Cordyceps militaris samples were related to their geographical origins.
Conclusion: SCoT markers are informative and could be used to detect polymorphism for medicinal fungus of Cordyceps militaris strains.
期刊介绍：
"Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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