[Research on Chemical Constituents and Anti-tumor Activity of Eupatorium chinense].

中药材 Pub Date : 2016-08-01

Xiao-qin Yu, Cheng-xiong Liu, Kun Zou, Hai-bo He, Jun-zhi Wang, Yi-hang Wu, Ying-chao Xu

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Abstract

Objective: To study the chemical constituents and their anti-tumor activity of Eupatorium chinense. Methods: The chemical constituents were separated and purified by the normal phase silica gel column chromatography,preparative thin-layer chromatography,and preparative HPLC. Their structures were determined by various spectral data,their antitumor activity in vitro was determined by MTT assay. Results: Six compounds were isolated from the ethyl acetate extract of Eupatorium chinense,and the structures were identified as eupalinilide G( 1),8β-( 4’-hydroxytigloyloxy)-5-desoxy-8-desacyleuparotin( 2),3-( hydroxymethyl)-1,13,14,15-tetrahydroxy-7,11,15-trimethyl-2,6,10-hexadecatriene( 3),3-( hydroxymethyl)-1,13,15-trihydroxy-7,11,15-trimethyl-2,6,10-hexadecatrien-14-yl acetate( 4),eupafolin( 5) and hiyodorilactone B( 6). Compound 2 showed cytotoxicity against HGC-27 and B16 cancer cell lines with IC50 values of 4. 29 μg/m L and 5. 53 μg/m L,respectively.

Methods: The chemical constituents were separated and purified by the normal phase silica gel column chromatography,preparative thin-layer chromatography,and preparative HPLC. Their structures were determined by various spectral data,their antitumor activity in vitro was determined by MTT assay.

Results: Six compounds were isolated from the ethyl acetate extract of Eupatorium chinense,and the structures were identified as eupalinilide G( 1),8β-( 4’-hydroxytigloyloxy)-5-desoxy-8-desacyleuparotin( 2),3-( hydroxymethyl)-1,13,14,15-tetrahydroxy-7,11,15-trimethyl-2,6,10-hexadecatriene( 3),3-( hydroxymethyl)-1,13,15-trihydroxy-7,11,15-trimethyl-2,6,10-hexadecatrien-14-yl acetate( 4),eupafolin( 5) and hiyodorilactone B( 6). Compound 2 showed cytotoxicity against HGC-27 and B16 cancer cell lines with IC50 values of 4. 29 μg/m L and 5. 53 μg/m L,respectively.

Conclusion: Compounds 2 ~ 5 are isolated from the Eupatorium chinense for the first time,and compound 2 has significant cytotoxic activity against HGC-27 cell line.

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[中国泽兰的化学成分及抗肿瘤活性研究]。

目的:研究泽兰的化学成分及其抗肿瘤活性。方法:采用正相硅胶柱层析法、制备薄层层析法和制备高效液相色谱法对化学成分进行分离纯化。用各种光谱数据测定其结构，用MTT法测定其体外抗肿瘤活性。结果:从紫茎泽兰乙酸乙酯萃取物中分离得到6个化合物，结构鉴定为:真泽兰内酯G(1)、8β-(4′-羟基tigloyloxy)-5-去氧基-8-去氧基leuparpartin(2)、3-(羟甲基)-1,13,14,15-四羟基-7,11,15-三甲基-2,6,10-十六烷基癸烯(3)、3-(羟甲基)-1,13,15-三羟基-7,11,15-三甲基-2,6,10-十六烷基癸烯-14-乙酸酯(4)、真泽兰内酯(5)和hiyodorilactone B(6)。化合物2对HGC-27和B16癌细胞具有细胞毒性IC50值为4。29 μg/m L;53 μg/m L。方法:采用正相硅胶柱层析法、制备薄层层析法和制备高效液相色谱法对化学成分进行分离纯化。用各种光谱数据测定其结构，用MTT法测定其体外抗肿瘤活性。结果:从紫茎泽兰乙酸乙酯萃取物中分离得到6个化合物，结构鉴定为:真泽兰内酯G(1)、8β-(4′-羟基tigloyloxy)-5-去氧基-8-去氧基leuparpartin(2)、3-(羟甲基)-1,13,14,15-四羟基-7,11,15-三甲基-2,6,10-十六烷基癸烯(3)、3-(羟甲基)-1,13,15-三羟基-7,11,15-三甲基-2,6,10-十六烷基癸烯-14-乙酸酯(4)、真泽兰内酯(5)和hiyodorilactone B(6)。化合物2对HGC-27和B16癌细胞具有细胞毒性IC50值为4。29 μg/m L;53 μg/m L。结论:化合物2 ~ 5为首次从紫茎泽兰中分离得到，化合物2对HGC-27细胞系具有明显的细胞毒活性。

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中药材

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期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.