[Optimization of Extracting Technology of Saponins in Steamed Panax notoginseng by Box-Behnken Design and Response Surface Methodology].

Abstract

Objective: To optimize the extraction process of saponins in steamed Panax notoginseng by Box-Behnken design-response surface methodology.

Methods: On the basis of single factor screening, Box-Behnken experimental design was employed,the content of saponins and dry extract rate of overall desirability（ OD） were as the evaluation index,and the extraction time（ X1）,solvent multiple（ X2）,solvent concentration（ X3） and extraction times were used as independent variables. Optimum experimental condition were selected from the stationary point of the response surfaces.

Results: By Box-Behnken design-response surface methodology and prognostic scoring system optimized scheme,the preferred processing condition were as follows, the right amount of steamed Panax notoginseng,added 16 times of 70% ethyl alcohol,extracted three times,and each time extracted for 2. 5 h. The calculated value of the process condition was basically consistent with the theoretical value,with less deviation.

Conclusion: The extraction technology of saponins optimized by Box-Behnken design is practical and rational,which can better predict the content of saponins and dry extract rate and other indicators in steamed Panax notoginseng.