[Identification on Five Kinds of Easily Confused Bupleurum Medicinal Materials].

中药材 Pub Date : 2016-09-01

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Abstract

Objective: Macroscopic identification, thin layer chromatography( TLC) identification and molecular identification based on the Chinese Pharmacopoeia were established to identify five kinds of Bupleurum medicinal materials such as Bupleurum chinense,Bupleurum scorzonerifolium, Bupleurum marginatum, Bupleurum marginatum var. stenophyllum and Bupleurum bicaule,which were easily confused.

Methods: According to the Chinese Pharmacopoeia,macroscopic characters of these five kinds of Bupleurum medicinal materials were observed and described; TLC identification method with saikosaponin d and control medicinal material of Bupleuri Radix as reference substances was established. Furthermore, DNA barcode was applied in identification of these confused drugs.

Results: Five Bupleurum medicinal materials could be easily distinguished by their morphological characters. Moreover, TLC of Bupleurum chinense,Bupleurum scorzonerifolium, Bupleurum marginatum and Bupleurum marginatum var. stenophyllum were extremely similar while being different from that of Bupleurum bicaule. The ITS2 sequences could be used to identify the five Bupleurum medicinal materials.

Conclusion: The established macroscopic identification and TLC for distinguishing of the five kinds of Bupleurum medicinal materials are simple and accurate while DNA barcode technology being effective and extensive. Besides, this study provides scientific basis for the further pharmacodynamic studies on Bupleurum marginatum and Bupleurum marginatum var. stenophyllum.

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【柴胡属五种易混淆药材鉴别】。

目的:建立基于《中国药典》的宏观鉴别、薄层色谱鉴别和分子鉴别方法，对中国柴胡、黄柴胡、边缘柴胡、窄叶柴胡、小柴胡等5种易混淆的柴胡属药材进行鉴别。方法:根据《中国药典》对这五种柴胡药材的宏观性状进行观察和描述;建立了以柴胡皂苷d和柴胡对照药材为对照品的薄层色谱鉴别方法。此外，将DNA条形码应用于这些混淆药物的鉴定。结果:柴胡属5种药材均可通过形态特征进行区分。此外，中国柴胡、柴胡、边缘柴胡、窄叶柴胡的薄层色谱极为相似，而与双叶柴胡的薄层色谱存在差异。ITS2序列可用于柴胡属5种药材的鉴定。结论:所建立的柴胡五种药材的宏观鉴别和薄层色谱鉴别方法简便、准确，DNA条形码技术有效、广泛。此外，本研究也为进一步开展柴胡和窄叶柴胡的药效学研究提供了科学依据。

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.