Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen
{"title":"Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation.","authors":"Amit Kr Chakraborty, Priyanka Mukherjee, Amarjit Karam, Samir Das, Luit Barkalita, Kekungo Puro, Rajkumari Sanjukta, Sandeep Ghatak, Ingudam Sakuntala, Ram Gopal Laha, Prabodh Borah, S V Ngachan, Indu Sharma, Arnab Sen","doi":"10.2174/1874357901812010110","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.</p><p><strong>Methods and material: </strong>The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.</p><p><strong>Results: </strong>Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.</p>","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"12 ","pages":"110-120"},"PeriodicalIF":0.0000,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142659/pdf/","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Virology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874357901812010110","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Introduction: The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.
Methods and material: The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.
Results: Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.