Viral Vectors for Gene Transfer

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-11-28 DOI:10.1002/cpmo.58
Yong Hong Chen, Megan S. Keiser, Beverly L. Davidson
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引用次数: 57

Abstract

Viral vectors are a promising tool for effective delivery of genetic material into cells. They take advantage of the natural ability of a virus to deliver a genetic payload into cells while being genetically modified such that their ability to replicate is crippled or removed. Here, an updated overview of routinely used viral vectors, including adeno-associated viruses (AAV), retroviruses/lentiviruses, and adenoviruses (Ads), is provided, as well as perspectives on their advantages and disadvantages in research and gene therapy. © 2018 by John Wiley & Sons, Inc.

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基因转移的病毒载体
病毒载体是一种很有前途的工具,可以有效地将遗传物质传递到细胞中。它们利用病毒的自然能力,将遗传载荷传递到细胞中,同时对其进行基因改造,使其复制能力受到削弱或消除。本文对常规使用的病毒载体,包括腺相关病毒(AAV)、逆转录病毒/慢病毒和腺病毒(Ads)进行了最新综述,并对其在研究和基因治疗中的优缺点进行了展望。©2018 by John Wiley &儿子,Inc。
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Current protocols in mouse biology
Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology
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期刊介绍: Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.
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