Key factors identified by proteomic analysis in maize (Zea mays L.) seedlings' response to long-term exposure to different phosphate levels.

IF 2.1 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS Proteome Science Pub Date : 2018-11-20 eCollection Date: 2018-01-01 DOI:10.1186/s12953-018-0147-3

Yanling Sun, Chunhua Mu, Xia Liu

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引用次数: 9

Abstract

Background: Maize seedlings are constantly exposed to inorganic phosphate (Pi)-limited environments. To understand how maize cope with low Pi (LP) and high Pi (HP) conditions, physiological and global proteomic analysis of QXN233 genotype were performed under the long-term Pi starvation and supplementation.

Methods: We investigated the physiological response of QXN233 genotype to LP and HP conditions and detected the changes in ion fluxes by non-invasive micro-test technology and gene expression by quantitative real-time polymerase chain reaction. QXN233 was further assessed using vermiculite assay, and then proteins were isolated and identified by nano-liquid chromatography-mass spectrometry.

Results: A negative relationship was observed between Na⁺ and Pi, and Na⁺ efflux was enhanced under HP condition. Furthermore, a total of 681 and 1374 were identified in the leaves and roots, respectively, which were mostly involved in metabolism, ion transport, and stress response. Importantly, several key Pi transporters were identified for breeding potential. Several ion transporters demonstrated an elaborate interplay between Pi and other ions, together contributing to the growth of QXN233 seedlings.

Conclusion: The results from this study provide insights into the response of maize seedlings to long-term Pi exposure.

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玉米(Zea mays L.)幼苗对不同磷水平长期暴露响应的蛋白质组学分析鉴定关键因素。

背景:玉米幼苗经常暴露在无机磷酸盐(Pi)有限的环境中。为了了解玉米如何应对低Pi (LP)和高Pi (HP)条件，我们对QXN233基因型在长期Pi饥饿和补充条件下进行了生理和全球蛋白质组学分析。方法:研究QXN233基因型在LP和HP条件下的生理反应，采用无创微检测技术检测离子通量的变化，采用实时定量聚合酶链反应检测基因表达。采用蛭石法对QXN233进行鉴定，然后采用纳米液相色谱-质谱法分离鉴定蛋白质。结果:Na+与Pi呈负相关，HP条件下Na+外排增强。在叶片和根中分别鉴定出681个和1374个，主要参与代谢、离子转运和胁迫响应。重要的是，几个关键的Pi转运蛋白被确定为育种潜力。几个离子转运体展示了Pi和其他离子之间复杂的相互作用，共同促进了QXN233幼苗的生长。结论:本研究结果揭示了玉米幼苗对Pi长期暴露的响应。

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来源期刊

Proteome Science 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： Proteome Science is an open access journal publishing research in the area of systems studies. Proteome Science considers manuscripts based on all aspects of functional and structural proteomics, genomics, metabolomics, systems analysis and metabiome analysis. It encourages the submissions of studies that use large-scale or systems analysis of biomolecules in a cellular, organismal and/or environmental context. Studies that describe novel biological or clinical insights as well as methods-focused studies that describe novel methods for the large-scale study of any and all biomolecules in cells and tissues, such as mass spectrometry, protein and nucleic acid microarrays, genomics, next-generation sequencing and computational algorithms and methods are all within the scope of Proteome Science, as are electron topography, structural methods, proteogenomics, chemical proteomics, stem cell proteomics, organelle proteomics, plant and microbial proteomics. In spite of its name, Proteome Science considers all aspects of large-scale and systems studies because ultimately any mechanism that results in genomic and metabolomic changes will affect or be affected by the proteome. To reflect this intrinsic relationship of biological systems, Proteome Science will consider all such articles.