Next generation sequencing characterizes HLA diversity in a registry population from the Netherlands

IF 4.1 4区医学 Q2 CELL BIOLOGY HLA Pub Date : 2019-03-24 DOI:10.1111/tan.13535

Lihua Hou, Elizabeth Enriquez, Misti Persaud, Noriko Steiner, Machteld Oudshoorn, Carolyn Katovich Hurley

引用次数: 19

Abstract

Next generation DNA sequencing is used to determine the HLA-A, -B, -C, -DRB1, -DRB3/4/5, and -DQB1 assignments of 1009 unrelated volunteers for the unrelated donor registry in The Netherlands. The analysis characterizes all HLA exons and introns for class I alleles; at least exons 2 to 3 for HLA-DRB1; and exons 2 to 6 for HLA-DQB1. Of the distinct alleles present, there are 229 class I and 71 class II; 36 of these alleles are novel. The majority (approximately 98%) of the cumulative allele frequency at each locus is contributed by alleles that appear three or more times. Alleles encoding protein variation outside of the antigen recognition domains are 0.6% of the class I assignments and 5.3% of the class II assignments.

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下一代测序特征HLA多样性登记人群来自荷兰

下一代DNA测序用于确定荷兰1009名非亲属志愿者的HLA-A， -B， -C， -DRB1， -DRB3/4/5和-DQB1分配。分析了HLA I类等位基因的所有外显子和内含子;HLA-DRB1至少外显子2至3;HLA-DQB1外显子2 ~ 6。在不同等位基因中，ⅰ类基因229个，ⅱ类基因71个;这些等位基因中有36个是新的。每个基因座累积等位基因频率的大部分(约98%)是由出现三次或三次以上的等位基因贡献的。在抗原识别结构域之外编码蛋白质变异的等位基因占I类配位的0.6%，占II类配位的5.3%。

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来源期刊

HLA Immunology and Microbiology-Immunology

CiteScore

3.00

自引率

28.80%

发文量

368

期刊介绍： HLA, the journal, publishes articles on various aspects of immunogenetics. These include the immunogenetics of cell surface antigens, the ontogeny and phylogeny of the immune system, the immunogenetics of cell interactions, the functional aspects of cell surface molecules and their natural ligands, and the role of tissue antigens in immune reactions. Additionally, the journal covers experimental and clinical transplantation, the relationships between normal tissue antigens and tumor-associated antigens, the genetic control of immune response and disease susceptibility, and the biochemistry and molecular biology of alloantigens and leukocyte differentiation. Manuscripts on molecules expressed on lymphoid cells, myeloid cells, platelets, and non-lineage-restricted antigens are welcomed. Lastly, the journal focuses on the immunogenetics of histocompatibility antigens in both humans and experimental animals, including their tissue distribution, regulation, and expression in normal and malignant cells, as well as the use of antigens as markers for disease.

期刊最新文献

Nomenclature for Factors of the HLA System, 2026. Identification of the Novel HLA-B Allele, HLA-B*37:122, in an Individual From Western India. Characterisation of the Novel HLA-B*18:264 Allele by Sequencing-Based Typing. Characterisation of the Novel HLA-B*35:02:25 Allele by Sequencing-Based Typing. Confirmation of the HLA-DPA1*01:03:59 Allele by Sequencing-Based Typing.