Magali Devriese, Jar How Lee, Tina Meng, Thoa Nong, Neng Jen Remi Shih, François A Lemonnier, Hiroko Miyadera, Lisa Giraldo, Cédric Usureau, Olivier Toutirais, Dave Lowe, Jean Luc Taupin
Eplet 52SK is unique in the HLA eplet registry as targeting the whole family of DQA1*01 alleles. It is proposed as an antibody-verified eplet but has not been validated enough to deserve this label. Especially, confusion can occur with reactivity targeting the 52PQ eplet which is present on the DQB1*05 and DQB1*06 alleles families, as DQ molecule stability imposes DQA1*01 to selectively associate with these DQ-β families only. Using two Luminex single antigen (LSA) assays from two vendors, beads bearing DR-α/DQ6 heterodimers, a special build LSA panel of additional DQ beads, and an adsorption/elution strategy relying on cells from deceased donors or recombinant cells solely expressing one DQ antigen, we definitely established the antibody-verified status of eplet 52SK using patients' sera reacting only against the DQ5 and DQ6 beads of the One Lambda LSA panel in routine patients' follow up. We also show that reactivity against this eplet is not a rare event among anti-DQ1 immunisation. This study further strengthens the importance of considering the DQA1 locus in immunological studies of HLA and in organ allocation strategies.
{"title":"Characterisation of the HLA-DQ alpha eplet 52SK targeting the DQA1*01 alleles family.","authors":"Magali Devriese, Jar How Lee, Tina Meng, Thoa Nong, Neng Jen Remi Shih, François A Lemonnier, Hiroko Miyadera, Lisa Giraldo, Cédric Usureau, Olivier Toutirais, Dave Lowe, Jean Luc Taupin","doi":"10.1111/tan.15700","DOIUrl":"10.1111/tan.15700","url":null,"abstract":"<p><p>Eplet 52SK is unique in the HLA eplet registry as targeting the whole family of DQA1*01 alleles. It is proposed as an antibody-verified eplet but has not been validated enough to deserve this label. Especially, confusion can occur with reactivity targeting the 52PQ eplet which is present on the DQB1*05 and DQB1*06 alleles families, as DQ molecule stability imposes DQA1*01 to selectively associate with these DQ-β families only. Using two Luminex single antigen (LSA) assays from two vendors, beads bearing DR-α/DQ6 heterodimers, a special build LSA panel of additional DQ beads, and an adsorption/elution strategy relying on cells from deceased donors or recombinant cells solely expressing one DQ antigen, we definitely established the antibody-verified status of eplet 52SK using patients' sera reacting only against the DQ5 and DQ6 beads of the One Lambda LSA panel in routine patients' follow up. We also show that reactivity against this eplet is not a rare event among anti-DQ1 immunisation. This study further strengthens the importance of considering the DQA1 locus in immunological studies of HLA and in organ allocation strategies.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterisation of the novel HLA-C*01:262 allele by sequencing-based typing.","authors":"Thibault Pajot, Vincent Elsermans, Joan Bitan, Isabelle Top, Myriam Labalette","doi":"10.1111/tan.15704","DOIUrl":"10.1111/tan.15704","url":null,"abstract":"<p><p>HLA-C*01:262 differs from HLA-C*01:02:01:01 by one nucleotide substitution in codon 150 in exon 3.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sudan Tao, Xuan You, Paul J Norman, Katherine M Kichula, Lina Dong, Nanying Chen, Ji He, Wei Zhang, Faming Zhu
Killer-cell immunoglobulin-like receptor (KIR) interactions with HLA class I have crucial roles in modulating NK cell function in response to viral infections. To explore the correlation between KIR/HLA and susceptibility to SARS-CoV-2 infection, we analysed polymorphism of KIR genes, haplotypes, HLA allotypes, and the interplay between KIR and HLA in individuals diagnosed with COVID-19. Compared to a population control group, we observed a significantly increased frequency of KIR3DL3*00802 in the COVID-19 group. When encoded by the HLA-B gene, the frequency of HLA-Bw4, a ligand for KIR3DL1, was at lower frequency in the COVID-19 group. Additionally, significantly elevated frequencies of KIR-Bx3, KIR3DL3*00301, 3DL3*048, and C1+HLA-C were identified in the COVID-19 group before multiple test correction, suggesting associations with susceptibility to SARS-CoV-2 infection. Our findings indicate that the KIR3DL3*00802 allele may be a high-risk factor for SARS-CoV-2 infection, while Bw4 encoded by HLA-B gene may confer protective effects against the infection.
杀伤细胞免疫球蛋白样受体（KIR）与 HLA I 类的相互作用在调节 NK 细胞功能以应对病毒感染方面起着至关重要的作用。为了探索 KIR/HLA 与 SARS-CoV-2 感染易感性之间的相关性，我们分析了 COVID-19 诊断个体中 KIR 基因的多态性、单倍型、HLA 异型以及 KIR 和 HLA 之间的相互作用。与人群对照组相比，我们观察到 COVID-19 组中 KIR3DL3*00802 的频率明显增加。由 HLA-B 基因编码的 HLA-Bw4（KIR3DL1 的配体）在 COVID-19 组中的频率较低。此外，在多重检验校正前，COVID-19 组中 KIR-Bx3、KIR3DL3*00301、3DL3*048 和 C1+HLA-C 的频率明显升高，这表明它们与 SARS-CoV-2 感染的易感性有关。我们的研究结果表明，KIR3DL3*00802等位基因可能是感染SARS-CoV-2的高危因素，而HLA-B基因编码的Bw4可能对感染具有保护作用。
{"title":"Analysis of KIR and HLA Polymorphism in Chinese Individuals With COVID-19.","authors":"Sudan Tao, Xuan You, Paul J Norman, Katherine M Kichula, Lina Dong, Nanying Chen, Ji He, Wei Zhang, Faming Zhu","doi":"10.1111/tan.15715","DOIUrl":"10.1111/tan.15715","url":null,"abstract":"<p><p>Killer-cell immunoglobulin-like receptor (KIR) interactions with HLA class I have crucial roles in modulating NK cell function in response to viral infections. To explore the correlation between KIR/HLA and susceptibility to SARS-CoV-2 infection, we analysed polymorphism of KIR genes, haplotypes, HLA allotypes, and the interplay between KIR and HLA in individuals diagnosed with COVID-19. Compared to a population control group, we observed a significantly increased frequency of KIR3DL3*00802 in the COVID-19 group. When encoded by the HLA-B gene, the frequency of HLA-Bw4, a ligand for KIR3DL1, was at lower frequency in the COVID-19 group. Additionally, significantly elevated frequencies of KIR-Bx3, KIR3DL3*00301, 3DL3*048, and C1<sup>+</sup>HLA-C were identified in the COVID-19 group before multiple test correction, suggesting associations with susceptibility to SARS-CoV-2 infection. Our findings indicate that the KIR3DL3*00802 allele may be a high-risk factor for SARS-CoV-2 infection, while Bw4 encoded by HLA-B gene may confer protective effects against the infection.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of the Novel HLA-C*07:01:126 Allele by Sequencing-Based Typing.","authors":"Vincent Elsermans, Jonathan Visentin, Thibault Pajot, Isabelle Top, Myriam Labalette","doi":"10.1111/tan.15713","DOIUrl":"https://doi.org/10.1111/tan.15713","url":null,"abstract":"<p><p>HLA-C*07:01:126 differs from HLA-C*07:01:01:01 by one nucleotide substitution in codon 328 in exon 7.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of the Novel HLA-A*24:02:169 Allele by Sequencing-Based Typing.","authors":"Vincent Elsermans, Marine Cargou, Thibault Pajot, Isabelle Top, Myriam Labalette","doi":"10.1111/tan.15712","DOIUrl":"https://doi.org/10.1111/tan.15712","url":null,"abstract":"<p><p>HLA-A*24:02:169 differs from HLA-A*24:02:01:01 by one nucleotide substitution in codon -23 in exon 1.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of the HLA-B*40:78 allele in a Taiwanese individual.","authors":"Kuo-Liang Yang, Py-Yu Lin","doi":"10.1111/tan.15709","DOIUrl":"10.1111/tan.15709","url":null,"abstract":"<p><p>One nucleotide substitution in codon 136 of HLA-B*40:02:01:01 results in a novel allele, HLA-B*40:78.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of three new HLA-A intronic variants by next-generation sequencing.","authors":"María Rosa Moya-Quiles, Manuel Muro","doi":"10.1111/tan.15708","DOIUrl":"10.1111/tan.15708","url":null,"abstract":"<p><p>Three novel HLA-A intronic variants, HLA-A*03:01:01:121, HLA-A*29:02:01:41 and HLA-A*30:02:01:24, detected by next-generation sequencing.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The HLA-C*14:152 Allele Was Identified Using a Next-Generation Sequencing Method.","authors":"Si-Hwan Kim, John Jeongseok Yang, Heung-Bum Oh","doi":"10.1111/tan.15714","DOIUrl":"https://doi.org/10.1111/tan.15714","url":null,"abstract":"<p><p>HLA-C*14:152 differs from HLA-C*14:02:01:01 by a non-synonymous nucleotide substitution in exon 5.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Francisco Javier Gil-Etayo, Daniel Arroyo-Sánchez, Jairo Eduardo Niño Ramírez, Pilar Terradillos-Sánchez, Amalia Tejeda Velarde
A single nucleotide substitution in the exon 3 gives rise to the novel HLA-DQA1*05:73 allele.
外显子 3 中的单核苷酸替换产生了新型 HLA-DQA1*05:73 等位基因。
{"title":"Detection of the novel HLA-DQA1*05:73 allele with an amino acid substitution in the α2 extracellular domain","authors":"Francisco Javier Gil-Etayo, Daniel Arroyo-Sánchez, Jairo Eduardo Niño Ramírez, Pilar Terradillos-Sánchez, Amalia Tejeda Velarde","doi":"10.1111/tan.15707","DOIUrl":"https://doi.org/10.1111/tan.15707","url":null,"abstract":"<p>A single nucleotide substitution in the exon 3 gives rise to the novel <i>HLA-DQA1*05:73</i> allele.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel HLA-DQB1*03 variant allele, HLA-DQB1*03:01:01:64, identified by next-generation sequencing","authors":"Diamanto Kouniaki, Vasiliki Kitsiou, Alexandra Tsirogianni","doi":"10.1111/tan.15705","DOIUrl":"10.1111/tan.15705","url":null,"abstract":"<p>The <i>HLA-DQB1*03:01:01:64</i> allele differs from <i>HLA-DQB1*03:01:01:03</i> by a single nucleotide substitution in intron 2.</p>","PeriodicalId":13172,"journal":{"name":"HLA","volume":null,"pages":null},"PeriodicalIF":5.9,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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