Mutans Streptococci counts from saliva and its protein profile in early childhood caries.

Q2 Medicine Interventional Medicine and Applied Science Pub Date : 2018-12-01 DOI:10.1556/1646.10.2018.42

Endang W Bachtiar, Ferry P Gultom, Atika Rahmasari, Boy M Bachtiar

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Abstract

Aim: This study aims to analyze the number Mutans Streptococci (MS) and its protein profile from the saliva of early childhood caries (ECC) and caries-free subjects.

Methods: MS counts were cultured from saliva samples, and the protein profile of MS was determined from ECC and caries-free subjects. The number of colonies were counted, and the protein bands with the molecular weight of 13, 29, 39, 41.3, 74, and 95 kDa were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis method.

Results: We found that the number of colonies from saliva of ECC patients was higher than those caries-free (22.20 × 106 CFU/ml vs. 19.16 × 106 CFU/ml, p < 0.05). There are higher expression frequencies in protein 29, 39, 41.3, and 74 kDa of MS in ECC than caries-free subjects.

Conclusions: There is the higher number of MS colonies and difference of MS protein profile isolated from saliva among children with ECC and caries-free counterparts.

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幼儿龋齿患者唾液中的突变链球菌数量及其蛋白质谱。

目的：本研究旨在分析儿童早期龋齿（ECC）和无龋受试者唾液中的突变链球菌（MS）数量及其蛋白质谱：方法：培养唾液样本中的MS菌落数，并测定ECC和无龋受试者唾液中MS的蛋白质谱。计数菌落数，用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法测定分子量为 13、29、39、41.3、74 和 95 kDa 的蛋白质条带：我们发现，ECC 患者唾液中的菌落数高于无龋患者（22.20 × 106 CFU/ml vs. 19.16 × 106 CFU/ml，p 结论：ECC 患者唾液中的 MS 菌落数高于无龋患者（22.20 × 106 CFU/ml vs. 19.16 × 106 CFU/ml，p）：ECC患儿和无龋患儿唾液中分离出的MS菌落数较高，MS蛋白图谱也存在差异。

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