{"title":"A Reliable Preclinical Model to Study the Impact of Cigarette Smoke in Development and Disease","authors":"Geraldine Aedo, Miguel Miranda, Myra N. Chávez, Miguel L. Allende, José T. Egaña","doi":"10.1002/cptx.78","DOIUrl":null,"url":null,"abstract":"<p>The World Health Organization has estimated that, worldwide, cigarette smoking has caused more than 100 million deaths in the last century, a number that is expected to increase in the future. Understanding cigarette smoke toxicity is key for research and development of proper public health policies. The current challenge is to establish a reliable preclinical model to evaluate the effects of cigarette smoke. In this work, we describe a simple method that allows for quantifying the toxic effects of cigarette smoke using zebrafish. Here, viability of larvae and adult fish, as well as the effects of cigarette smoke extracts on vascular development and tissue regeneration, can be easily assayed. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"80 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.78","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.78","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
The World Health Organization has estimated that, worldwide, cigarette smoking has caused more than 100 million deaths in the last century, a number that is expected to increase in the future. Understanding cigarette smoke toxicity is key for research and development of proper public health policies. The current challenge is to establish a reliable preclinical model to evaluate the effects of cigarette smoke. In this work, we describe a simple method that allows for quantifying the toxic effects of cigarette smoke using zebrafish. Here, viability of larvae and adult fish, as well as the effects of cigarette smoke extracts on vascular development and tissue regeneration, can be easily assayed. © 2019 by John Wiley & Sons, Inc.
研究吸烟对发育和疾病影响的可靠临床前模型
世界卫生组织估计,在上个世纪,吸烟在世界范围内造成了1亿多人死亡,预计这一数字今后还会增加。了解香烟烟雾的毒性是研究和制定适当的公共卫生政策的关键。目前的挑战是建立一个可靠的临床前模型来评估香烟烟雾的影响。在这项工作中,我们描述了一种简单的方法,可以用斑马鱼来量化香烟烟雾的毒性作用。在这里,可以很容易地测定幼鱼和成鱼的活力,以及香烟烟雾提取物对维管发育和组织再生的影响。©2019 by John Wiley &儿子,Inc。
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