{"title":"Isolation of Alveolar Type II Cells from Adult Bovine Lung","authors":"Diane Frances Lee, Mark Andrew Chambers","doi":"10.1002/cptx.71","DOIUrl":null,"url":null,"abstract":"<p>Alveolar type II (ATII) cells play a key role as part of the distal lung epithelium, including in the innate immune response and as self-renewing progenitors to replace alveolar type I (ATI) cells during epithelial regeneration. Their secretion of surfactant protein helps maintain homeostasis and exerts protective, antimicrobial properties. ATII cells remain difficult to study, partly due to inefficient and expensive isolation methods, a propensity to differentiate into ATI cells, and susceptibility to fibroblast contamination. Published methods of isolation often require specialized technology, negatively impacting the development of <i>in vitro</i> models of disease, including bovine tuberculosis. Presented here is a simple and cost-effective method for generation of bovine primary ATII cells. These cells exhibit an ATII phenotype in 2D and 3D culture and are conducive to further study of the role of ATII cells in bovine respiratory diseases. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"80 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.71","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.71","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Alveolar type II (ATII) cells play a key role as part of the distal lung epithelium, including in the innate immune response and as self-renewing progenitors to replace alveolar type I (ATI) cells during epithelial regeneration. Their secretion of surfactant protein helps maintain homeostasis and exerts protective, antimicrobial properties. ATII cells remain difficult to study, partly due to inefficient and expensive isolation methods, a propensity to differentiate into ATI cells, and susceptibility to fibroblast contamination. Published methods of isolation often require specialized technology, negatively impacting the development of in vitro models of disease, including bovine tuberculosis. Presented here is a simple and cost-effective method for generation of bovine primary ATII cells. These cells exhibit an ATII phenotype in 2D and 3D culture and are conducive to further study of the role of ATII cells in bovine respiratory diseases. © 2019 by John Wiley & Sons, Inc.
成年牛肺ⅱ型肺泡细胞的分离
肺泡II型(ATII)细胞作为远端肺上皮的一部分发挥着关键作用,包括在先天免疫反应中,以及在上皮再生过程中作为自我更新的祖细胞取代肺泡I型(ATI)细胞。它们分泌表面活性剂蛋白有助于维持体内平衡,并发挥保护和抗菌作用。ATII细胞仍然难以研究,部分原因是分离方法效率低下且昂贵,容易分化为ATI细胞,易受成纤维细胞污染。已发表的分离方法往往需要专门的技术,这对包括牛结核病在内的疾病体外模型的发展产生了负面影响。这里提出了一种简单而经济有效的方法来产生牛原代ATII细胞。这些细胞在2D和3D培养中表现出ATII表型,有助于进一步研究ATII细胞在牛呼吸道疾病中的作用。©2019 by John Wiley &儿子,Inc。
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