Chemical Composition and Biological Activities of Lebanese Pentapera Plant.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2019-03-14 DOI:10.12659/MSMBR.914741

Hany Kallassy, Hussein Fayyad-Kazan, Rawan Makki, Monya Kaeen, Amer Sakr, Osama Alyamani, Rim El Dirani, Eva Hamade, Mohammad Fayyad-Kazan, Bassam Badran

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引用次数: 2

Abstract

BACKGROUND Due to their chemical constituents and biological properties, plants have long been used to control life-threatening diseases. The flora of Lebanon includes many plants that have already been demonstrated to have medicinal value, and other species, such as Pentapera sicula libanotica, that are yet to be characterized. The present study characterized the chemical composition, anti-oxidant, anti-inflammatory, and anti-proliferative potential of aqueous, ethanol, and methanol extracts derived from the leaves of the Lebanese Pentapera plant. MATERIAL AND METHODS High-performance liquid chromatography (HPLC) was used to determine the chemical composition. Gas chromatography (GC) coupled with mass spectrometry (MS) was applied to determine the content of essential oil. DPPH radical scavenging assay was performed to evaluate the anti-oxidant potential. The anti-inflammatory potential was assessed using quantitative real-time PCR (qRT-PCR) by measuring TNF-alpha, IL-6, and CCL4 mRNA levels, and we assessed Cox-2 and iNOS proteins levels using Western blot (WB) analysis. MTT assay was carried out to determine the anti-proliferative potential. RESULTS We identified, mainly in the alcoholic (methanol and ethanol) extracts, distinct bioactive compounds with pharmacological relevance. In parallel, with their phytochemical content, these 2 extracts showed significant anti-oxidant, anti-inflammatory and anti-proliferative capacities. CONCLUSIONS Pentapera sicula libanotica appears to be a promising pharmacological tool.

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黎巴嫩五芒菜属植物的化学成分及生物活性。

由于植物的化学成分和生物学特性，植物一直被用来控制威胁生命的疾病。黎巴嫩的植物群包括许多已被证明具有药用价值的植物，以及其他一些尚未被鉴定的物种，如黎巴嫩五冬葵。本研究表征了黎巴嫩五芒草叶片的水、乙醇和甲醇提取物的化学成分、抗氧化、抗炎和抗增殖潜力。材料与方法采用高效液相色谱法测定其化学成分。采用气相色谱-质谱联用技术测定挥发油的含量。采用DPPH自由基清除实验评价其抗氧化能力。采用实时荧光定量PCR (qRT-PCR)检测tnf - α、IL-6和CCL4 mRNA水平，采用Western blot (WB)分析Cox-2和iNOS蛋白水平。采用MTT法测定其抗增殖潜能。结果:我们主要在酒精(甲醇和乙醇)提取物中鉴定出具有药理相关性的独特生物活性化合物。与此同时，这两种提取物具有显著的抗氧化、抗炎和抗增殖能力。结论:五瓣草是一种很有前景的药理工具。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.