Synergistic Effect of Polyalthia longifolia Leaf and Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) by Microscopic Technique.

Q2 Medicine Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2020-01-01 DOI:10.2174/1871523018666190522112902

Balasupramaniam Kirubakari, Yeng Chen, Sreenivasan Sasidharan

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引用次数: 4

Abstract

Background: Polyalthia longifolia is a popular medicinal plant and has been widely used as a traditional remedy for centuries in curing of various ailments. The purpose of this study was conducted to determine the in situ antimicrobial synergistic effects between Polyalthia longifolia leaf ethyl acetate fraction (PLEAF) and ampicillin against MRSA local isolate by using modern microscopy technique.

Methods: Hence, the evaluation of the synergistic activity of PLEAF and ampicillin against MRSA local isolate was conducted with scanning electron microscopy (SEM).

Results: The combinational effect of PLEAF fraction and ampicillin exhibited significant antibacterial activity against MRSA. Bacterial cells observations showed invagination, impaired cell division, extensive wrinkles, cell shrinkage, the appearance of a rougher cell with fibrous matrix and clustered cells which confirmed the synergistic effect of PLEAF and ampicillin against MRSA local isolate by SEM.

Conclusion: Conclusively, the in situ SEM observation proved the synergistic antimicrobial activity between PLEAF fraction and ampicillin to destroy the MRSA resistance bacteria which is an important aspect of PLEAF fraction to be used in the future combinational therapy.

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长叶蓼叶与抗生素对耐甲氧西林金黄色葡萄球菌(MRSA)的协同作用

背景:长叶蓼是一种流行的药用植物，几个世纪以来一直被广泛用于治疗各种疾病。本研究采用现代显微镜技术，研究长叶蓼叶乙酸乙酯部分(PLEAF)与氨苄西林对MRSA局部分离株的原位协同抑菌作用。方法:采用扫描电镜(SEM)评价PLEAF与氨苄西林对MRSA局部分离株的协同作用。结果:PLEAF组分与氨苄西林联用对MRSA具有明显的抑菌活性。细菌细胞观察显示内翻、细胞分裂受损、广泛皱褶、细胞收缩、出现纤维基质的粗糙细胞和聚集细胞，证实了PLEAF和氨苄西林对MRSA局部分离物的协同作用。结论:原位扫描电镜观察证实了PLEAF组分与氨苄西林具有协同抑菌作用，可杀灭MRSA耐药菌，这是PLEAF组分在今后联合治疗中应用的重要方面。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

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0.00%

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期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.