Anti-inflammatory Effects of First-line Anti-arthritic Drugs on T-cell Activation.

Q2 Medicine Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2023-01-01 DOI:10.2174/1871523022666230201144553

Nicholas Manolios, Guojiang Hou

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Abstract

Aim: The in vitro effects of commonly used first-line anti-arthritic drugs on early stages of T-cell activation were examined.

Methods: The 2B4.11 murine T cell hybridoma cell line recognizing pigeon cytochrome c (PCC) as the antigen was co-cultured with the histocompatible antigen presenting B cell hybridoma line LK35.2, PCC, and anti-arthritic drugs, including methotrexate, hydroxychloroquine, salazopyrine, cyclosporin, and leflunomide. After 16 hours of incubation, the supernatant was removed, and cytokines were assayed.

Results: Anti-arthritic drugs inhibited the production of pro-inflammatory cytokines IL-2, IL-6, IFN-γ, GM-CSF, and TNF-α (Th1 cytokines) to a varying extent. Surprisingly, leflunomide, salazopyrine, prednisone and indomethacin as well as blocking Th1 cytokines, stimulated the production of the anti-inflammatory cytokine IL-10, a Th2 cytokine.

Conclusion: Anti-arthritic medications can inhibit the production of pro-inflammatory cytokines and in some cases, incite a Th2 response that could potentially inhibit the progression of the immune response.

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一线抗关节炎药物对t细胞活化的抗炎作用

目的:观察常用一线抗关节炎药物对早期t细胞活化的体外影响。方法:将以鸽子细胞色素c (PCC)为抗原的2B4.11小鼠T细胞杂交瘤细胞株与组织相容性抗原LK35.2呈递B细胞杂交瘤细胞株、PCC和抗关节炎药物甲氨蝶呤、羟氯喹、萨拉唑比林、环孢素、来氟米特共培养。孵育16小时后，取上清液，检测细胞因子。结果:抗关节炎药物不同程度抑制促炎细胞因子IL-2、IL-6、IFN-γ、GM-CSF、TNF-α (Th1细胞因子)的产生。令人惊讶的是，来氟米特、萨拉吡林、强的松和吲哚美辛以及阻断Th1细胞因子，刺激了抗炎细胞因子IL-10 (Th2细胞因子)的产生。结论:抗关节炎药物可以抑制促炎细胞因子的产生，在某些情况下，可以激发Th2反应，从而潜在地抑制免疫反应的进展。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.