{"title":"Effects of Platelet-Derived Material (Platelet-Rich Fibrin) on Bone Regeneration.","authors":"Jae-Seek You, Su-Gwan Kim, Ji-Su Oh, Jae-Sung Kim","doi":"10.1097/ID.0000000000000877","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to evaluate the effects of the growth factor within platelet-rich fibrin (PRF) in proliferation and differentiation of osteoblast and to observe the effectiveness of PRF.</p><p><strong>Materials and methods: </strong>The colorimetric MTT assay, cell live and dead assay, alkaline phosphatase staining and activity assay, alizarine red S, and von Kossa staining were performed. Finally, the alterations of biomarkers associated with bone formation were verified at the mRNA level by quantitative polymerase chain reaction (PCR) and quantitative real-time PCR. In in vivo study, 6 adult mongrel dogs were used. The defect was performed and divided into 3 groups: (1) defect left unfilled, (2) defect filled with only 0.25-g Bio-Oss, and (3) defect filled with 0.25-g Bio-Oss mixed with PRF.</p><p><strong>Results: </strong>MTT and cell live and dead assay showed that PRF did not affect the cell viability in MG-63 cells. The alkaline phosphatase activity, calcification, and mineralization were gradually increased in the MG-63 cells treated with PRF. Furthermore, the mRNA levels of biomarker gene in the MG-63 cells treated with PRF were significantly higher than those of control. In in vivo study, both radiographical and histological evaluations showed that the new bone formations were significantly increased in the defecting bone region transplanted with Bio-Oss and PRF compared with Bio-Oss only at 2 weeks after transplantation.</p><p><strong>Conclusion: </strong>PRF can promote the bone regeneration without any complications.</p>","PeriodicalId":13309,"journal":{"name":"Implant Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/ID.0000000000000877","citationCount":"30","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Implant Dentistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/ID.0000000000000877","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 30
Abstract
Purpose: The purpose of this study was to evaluate the effects of the growth factor within platelet-rich fibrin (PRF) in proliferation and differentiation of osteoblast and to observe the effectiveness of PRF.
Materials and methods: The colorimetric MTT assay, cell live and dead assay, alkaline phosphatase staining and activity assay, alizarine red S, and von Kossa staining were performed. Finally, the alterations of biomarkers associated with bone formation were verified at the mRNA level by quantitative polymerase chain reaction (PCR) and quantitative real-time PCR. In in vivo study, 6 adult mongrel dogs were used. The defect was performed and divided into 3 groups: (1) defect left unfilled, (2) defect filled with only 0.25-g Bio-Oss, and (3) defect filled with 0.25-g Bio-Oss mixed with PRF.
Results: MTT and cell live and dead assay showed that PRF did not affect the cell viability in MG-63 cells. The alkaline phosphatase activity, calcification, and mineralization were gradually increased in the MG-63 cells treated with PRF. Furthermore, the mRNA levels of biomarker gene in the MG-63 cells treated with PRF were significantly higher than those of control. In in vivo study, both radiographical and histological evaluations showed that the new bone formations were significantly increased in the defecting bone region transplanted with Bio-Oss and PRF compared with Bio-Oss only at 2 weeks after transplantation.
Conclusion: PRF can promote the bone regeneration without any complications.
目的:探讨富血小板纤维蛋白(PRF)内生长因子对成骨细胞增殖分化的影响，并观察PRF的作用。材料和方法:比色MTT法、细胞活与死法、碱性磷酸酶染色及活性法、茜素红S法、von Kossa法。最后，通过定量聚合酶链反应(PCR)和实时定量PCR在mRNA水平上验证与骨形成相关的生物标志物的改变。在体内研究中，选用6只成年杂种狗。将缺损进行修复，分为3组:(1)缺损未填充，(2)缺损仅填充0.25 g Bio-Oss，(3)缺损填充0.25 g Bio-Oss与PRF混合。结果:MTT和细胞活死实验显示，PRF对MG-63细胞的细胞活力无影响。PRF对MG-63细胞碱性磷酸酶活性、钙化和矿化作用逐渐增强。此外，PRF处理MG-63细胞的生物标志物基因mRNA水平显著高于对照组。在体内研究中，x线和组织学评估显示，移植后2周，与Bio-Oss相比，Bio-Oss和PRF移植的缺损骨区域的新骨形成明显增加。结论:PRF能促进骨再生，无并发症。
期刊介绍：
Cessation. Implant Dentistry, an interdisciplinary forum for general practitioners, specialists, educators, and researchers, publishes relevant clinical, educational, and research articles that document current concepts of oral implantology in sections on biomaterials, clinical reports, oral and maxillofacial surgery, oral pathology, periodontics, prosthodontics, and research. The journal includes guest editorials, letters to the editor, book reviews, abstracts of current literature, and news of sponsoring societies.
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