Assessment of anti-cancer effects of koenimbine on colon cancer cells.

Q3 Medicine Human Antibodies Pub Date : 2020-01-01 DOI:10.3233/HAB-200405

Maliheh Astaneh, Soudeh Ghafouri-Fard, Zahra Fazeli, Zahra Taherian-Esfahani, Sepideh Dashti, Elahe Motevaseli

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引用次数: 3

Abstract

Background: Recent studies have highlighted the role of natural elements in reduction of cancer cell growth and apoptosis. Koenimbine, a natural product isolated from Murraya koenigii (L) Spreng is a substance with cytotoxic effects on cancer cells.

Aim: The effects of koenimbine on HT-29 and SW48 colon cancer cells were evaluated by MTT and Annexin V assays. Expression levels of Wnt/β-catenin pathway genes were quantified by real time PCR.

Results: The IC50 values of koenimbine in HT-29 and SW48 was calculated to be 50 μg/ml based on the results of MTT assay. This value was 75 μg/ml in IEC-18 cells which were used as normal control. Annexin V assays revealed induction of cell apoptosis and necrosis in HT-29 and SW48 cells but not IEG18 cells by koenimbine. Koenimbin treatment resulted in significant down-regulation of CYCLD1 expression in SW48 cell line, but up-regulation of this gene in HT29 cell line. Expression of TBLR1, DKK1, GSK3B and β-catenin was significantly decreased after koenimbin treatment in HT-19 cell line. Moreover, expression of DKK1 and GSK3B was significantly decreased after koenimbin treatment in SW-40 cell line. TCF4 expression was not detected in any of cell lines either before or after treatment with koenimbin.

Conclusion: The current in vitro study showed the cytotoxic effects of koenimbin on two colon cancer cell lines and the effects of this substance on expression of selected genes from Wnt-β catenin pathway. Future in vivo studies are needed before suggestion of this substance as an anti-cancer drug.

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柯尼滨对结肠癌细胞的抗癌作用评价。

背景:近年来的研究强调了天然元素在减少癌细胞生长和凋亡中的作用。Koenimbine是一种天然产物，从Murraya koenigii (L) spring中分离出来，是一种对癌细胞具有细胞毒性的物质。目的:采用MTT法和Annexin V法观察柯尼滨对HT-29和SW48结肠癌细胞的影响。real - time PCR检测Wnt/β-catenin通路基因的表达水平。结果:根据MTT法计算克尼宾在HT-29和SW48中的IC50值为50 μg/ml。以正常对照的IEC-18细胞为75 μg/ml。膜联蛋白V检测显示，koenimine在HT-29和SW48细胞中诱导细胞凋亡和坏死，但对IEG18细胞无诱导作用。Koenimbin处理导致SW48细胞系CYCLD1表达显著下调，而HT29细胞系CYCLD1表达上调。koenimbin处理后，HT-19细胞株中TBLR1、DKK1、GSK3B和β-catenin的表达显著降低。此外，koenimbin处理后SW-40细胞株中DKK1和GSK3B的表达显著降低。在koenimbin处理前后，没有检测到TCF4在任何细胞系中的表达。结论:目前的体外研究显示了koenimbin对两种结肠癌细胞系的细胞毒作用，以及该物质对Wnt-β catenin通路中选定基因表达的影响。在提出这种物质作为抗癌药物之前，还需要进一步的体内研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.