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{"title":"Aseptic Technique","authors":"Tomasz Bykowski, Brian Stevenson","doi":"10.1002/cpmc.98","DOIUrl":null,"url":null,"abstract":"<p>This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. © 2020 by John Wiley & Sons, Inc.</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":"56 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.98","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.98","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. © 2020 by John Wiley & Sons, Inc.
无菌技术
本文描述了可以降低培养物污染(败血症)风险的常见实验室程序，统称为“无菌技术”。描述了无菌工作的两个主要策略:使用本生灯和使用层流罩。这两种方法都以通用方案的形式呈现，适用于各种实验室任务，如移液和分配等分液，制备生长介质，接种，传代和在培养皿上传播微生物。©2020 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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