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{"title":"Estimation of Microbial Viability Using Flow Cytometry.","authors":"Hazel Davey, Stéphane Guyot","doi":"10.1002/cpcy.72","DOIUrl":null,"url":null,"abstract":"<p><p>For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold standard) usage equates viability and culturability (i.e., the ability to multiply) but the process of determining culturability is often too slow. Flow cytometry provides the opportunity to make rapid and quantitative measurements of dye uptake in large numbers of cells and we can therefore exploit the flow cytometric approach to evaluate so-called viability stains and to develop protocols for more routine assessments of microbial viability. This article provides a commentary and several protocols have been included to ensure that users have a firm basis for attempting these reasonably difficult assays on traditional flow cytometer instruments. What is clear is that each assay must be carefully validated with the particular microorganism of interest before being applied in any research, clinical, or service form. © 2020 The Authors.</p>","PeriodicalId":11020,"journal":{"name":"Current Protocols in Cytometry","volume":"93 1","pages":"e72"},"PeriodicalIF":0.0000,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpcy.72","citationCount":"43","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cytometry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/cpcy.72","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}
引用次数: 43
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Abstract
For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold standard) usage equates viability and culturability (i.e., the ability to multiply) but the process of determining culturability is often too slow. Flow cytometry provides the opportunity to make rapid and quantitative measurements of dye uptake in large numbers of cells and we can therefore exploit the flow cytometric approach to evaluate so-called viability stains and to develop protocols for more routine assessments of microbial viability. This article provides a commentary and several protocols have been included to ensure that users have a firm basis for attempting these reasonably difficult assays on traditional flow cytometer instruments. What is clear is that each assay must be carefully validated with the particular microorganism of interest before being applied in any research, clinical, or service form. © 2020 The Authors.
用流式细胞术估计微生物活力。
特别是对微生物来说,生存能力是一个难以定义的术语,因此也是一种难以测量的状态。传统的(和黄金标准)用法将生存能力和可培养性(即繁殖能力)等同起来,但确定可培养性的过程通常太慢。流式细胞术提供了在大量细胞中对染料摄取进行快速定量测量的机会,因此我们可以利用流式细胞术方法来评估所谓的活力染色,并制定更常规的微生物活力评估方案。本文提供了一个评论和几个协议已包括,以确保用户有一个坚实的基础,尝试这些合理的困难的分析在传统的流式细胞仪仪器。很清楚的是,在应用于任何研究、临床或服务形式之前,每个测定都必须与感兴趣的特定微生物仔细验证。©2020作者。
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