Nicotinic acetylcholine receptors (rs1051730) gene polymorphism and surfactant protein D level in chronic obstructive pulmonary disease.

Abstract

Chronic obstructive pulmonary disease (COPD) is a complicated disorder that may be genetically determined. It is possible that genetic determinants of nicotine addiction contribute to COPD burden. Surfactant protein D (SP-D) ensures correct ventilatory function . The aim of this work is to study serum level of (SP-D) and nicotinic acetylcholine receptors (nAChRs) (rs1051730) (G/A) gene polymorphism in COPD. We recruited 80 patients with COPD and 80 ages and gender matched healthy individuals. Estimation of Serum SP-D level (by ELISA) and genotyping of (nAChRs) (rs1051730) using TaqMan Allelic discrimination assay technique (real time PCR) were done for all individuals. Results showed significant increased serum SP-D level (P<0.001) and AA genotype and A allele frequencies in COPD patients (P< 0.001) with OR (CI) [6.15 (2.76-13.72) and 3.65 (2.29-5.78) respectively]. We conclude that AA genotype of nAChRs (rs1051730) and elevated serum level of SP-D might be risk factors for COPD.