Differences in the expression of the renin angiotensin system and the kallikrein-kinin system during the course of myocardial infarction in male and female Wistar rats.

IF 2.1 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2020-04-01 DOI:10.1177/1470320319900038
Jazmín Flores-Monroy, Diego Lezama-Martínez, Salvador Fonseca-Coronado, Luisa Martínez-Aguilar
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引用次数: 2

Abstract

Background: There is some evidence that components of the renin-angiotensin system and kallikrein-kinin system are not similarly regulated in both sexes. The aim of this work was to analyze the expression of angiotensin-converting enzyme, angiotensin-converting enzyme 2, angiotensin 1 receptor, angiotensin 2 receptor, beta-1 receptor, and beta-2 receptor during the evolution of myocardial infarction.

Methods: Thirty-six male and 36 female Wistar rats were used. Myocardial infarction was induced. Six groups of both sexes were formed, (n=6): (a) sham; (b) 48 h myocardial infarction; (c) one week myocardial infarction; (d) two weeks myocardial infarction; (e) three weeks myocardial infarction and (f) four weeks myocardial infarction. The expression was evaluated by real-time polymerase chain reaction on the penumbra of left ventricle.

Results: The mRNA expression of most biomarkers was lower in females than in males. During acute infarction, an increase of all protein expression was found in female and at two weeks while in the male only biomarker changes occurred at three weeks. In addition, in male biomarkers mRNA expression decreased during chronic infarction while in females it did not.

Conclusions: The renin-angiotensin system and kallikrein-kinin system biomarkers expression occurs at earlier times in the female than in the male rat. In addition, during chronic myocardial infarction these biomarkers remained unchanged in females while in males they decreased.

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雄性和雌性Wistar大鼠心肌梗死过程中肾素-血管紧张素系统和钾化钾-激肽系统表达的差异。
背景:有一些证据表明肾素-血管紧张素系统和钾化钾素-激肽系统的成分在两性中没有相似的调节。分析血管紧张素转换酶、血管紧张素转换酶2、血管紧张素1受体、血管紧张素2受体、β -1受体和β -2受体在心肌梗死演变过程中的表达。方法:雄性Wistar大鼠36只,雌性Wistar大鼠36只。诱导心肌梗死。男女共分为六组(n=6):(a)假手术;(b) 48 h心肌梗死;(c) 1周心肌梗死;(d) 2周心肌梗死;(e) 3周心肌梗死;(f) 4周心肌梗死。采用实时聚合酶链反应在左心室半暗区检测其表达。结果:大多数生物标志物的mRNA表达在女性中低于男性。在急性梗死期间,在女性和两周时发现所有蛋白质表达增加,而在男性中,只有生物标志物在三周时发生变化。此外,男性生物标志物mRNA表达在慢性梗死期间下降,而女性则没有。结论:雌性大鼠肾素-血管紧张素系统和钾化钾素-激肽系统生物标志物的表达早于雄性大鼠。此外,在慢性心肌梗死期间,这些生物标志物在女性中保持不变,而在男性中则有所下降。
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来源期刊
CiteScore
6.20
自引率
0.00%
发文量
16
审稿时长
6-12 weeks
期刊介绍: JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.
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