{"title":"Analysis of Busulfan in Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)","authors":"Abed Pablo, Autumn R. Breaud, William Clarke","doi":"10.1002/cptx.93","DOIUrl":null,"url":null,"abstract":"<p>Bone marrow transplantation is used to treat particular types of cancers such as lymphoma, leukemia, and multiple myeloma. Appropriate dosing of busulfan during the preparative phase is critical for a successful allograft; if blood concentrations get too high significant liver toxicity can occur, if blood concentrations are too low, then graft-versus-host disease (GVHD) can develop. Busulfan monitoring in blood allows hospitals with the opportunity to provide individualized medicine to patients and improve overall patient outcome. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) is an important analytical method for quantification of busulfan in plasma in order to optimize the dose. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol</b>: Analysis of busulfan by liquid chromatography/mass spectrometry</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"84 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.93","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.93","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Bone marrow transplantation is used to treat particular types of cancers such as lymphoma, leukemia, and multiple myeloma. Appropriate dosing of busulfan during the preparative phase is critical for a successful allograft; if blood concentrations get too high significant liver toxicity can occur, if blood concentrations are too low, then graft-versus-host disease (GVHD) can develop. Busulfan monitoring in blood allows hospitals with the opportunity to provide individualized medicine to patients and improve overall patient outcome. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) is an important analytical method for quantification of busulfan in plasma in order to optimize the dose. © 2020 Wiley Periodicals LLC.
Basic Protocol: Analysis of busulfan by liquid chromatography/mass spectrometry
液相色谱-串联质谱法(LC-MS/MS)分析血浆中布苏凡
骨髓移植用于治疗特定类型的癌症，如淋巴瘤、白血病和多发性骨髓瘤。在准备阶段适当剂量的丁硫丹对同种异体移植的成功至关重要;如果血药浓度过高，可发生显著的肝毒性，如果血药浓度过低，则可发展为移植物抗宿主病(GVHD)。血液中的Busulfan监测使医院有机会为患者提供个性化药物并改善患者的整体预后。液相色谱-串联质谱法(LC-MS/MS)是一种重要的定量分析方法，用于优化血浆中磺胺的剂量。©2020 Wiley Periodicals llc .基本方案:用液相色谱/质谱法分析丁硫丹
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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