In vitro analysis of the renin-angiotensin system and inflammatory gene transcripts in human bronchial epithelial cells after infection with severe acute respiratory syndrome coronavirus.

IF 2.1 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2020-04-01 DOI:10.1177/1470320320928872
Can Turk, Seyhan Turk, Elif Sena Temirci, Umit Yavuz Malkan, İbrahim C Haznedaroglu
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引用次数: 14

Abstract

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a recently identified coronavirus family member that triggers a respiratory disease similar to severe acute respiratory syndrome coronavirus (SARS-CoV). SARS-CoV and SARS-CoV-2 are very similar to each other in many respects, such as structure, genetics, and pathobiology. We hypothesized that coronaviruses could affect pulmonary tissues via integration with the critical immune genes after their interaction with renin-angiotensin system (RAS) elements. The aim of the present bioinformatics study was to assess expression changes of the RAS and non-RAS genes, particularly immune response genes, in the lung epithelial cells after infection with SARS-CoV.

Methods: Linear regression, hierarchical clustering, pathway analysis, and network analysis were performed using the E-GEOD-17400 data set.

Results: The whole-genome expression data of the lung epithelial cells infected with SARS-CoV for 12, 24, and 48 hours were analyzed, and a total of 15 RAS family and 29 immune genes were found to be highly correlated with the exposure time to the virus in the studied groups.

Conclusion: RAS genes are important at the initiation of the infections caused by coronavirus family members and may have a strong relationship with the exchange of immune genes in due course following the infection.

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冠状病毒感染后人支气管上皮细胞肾素-血管紧张素系统及炎症基因转录物的体外分析
简介:严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)是一种新发现的冠状病毒家族成员,可引发类似于严重急性呼吸综合征冠状病毒(SARS-CoV)的呼吸道疾病。SARS-CoV和SARS-CoV-2在结构、遗传学和病理生物学等许多方面非常相似。我们假设冠状病毒在与肾素-血管紧张素系统(RAS)元件相互作用后,通过与关键免疫基因的整合影响肺组织。本生物信息学研究的目的是评估感染sars冠状病毒后肺上皮细胞中RAS和非RAS基因,特别是免疫反应基因的表达变化。方法:采用E-GEOD-17400数据集进行线性回归、层次聚类、路径分析和网络分析。结果:对感染SARS-CoV 12、24和48 h肺上皮细胞全基因组表达数据进行分析,发现实验组中有15个RAS家族和29个免疫基因与病毒暴露时间高度相关。结论:RAS基因在冠状病毒感染开始时起重要作用,并可能与感染后适当的免疫基因交换有密切关系。
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来源期刊
CiteScore
6.20
自引率
0.00%
发文量
16
审稿时长
6-12 weeks
期刊介绍: JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.
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