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{"title":"Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays","authors":"David G. Christensen, Karen L. Visick","doi":"10.1002/cpmc.103","DOIUrl":null,"url":null,"abstract":"<p><i>Vibrio fischeri</i> is a nonpathogenic organism related to pathogenic <i>Vibrio</i> species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of <i>V. fischeri</i>, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of <i>V. fischeri</i>. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Growth of <i>V. fischeri</i> from frozen stocks</p><p><b>Basic Protocol 2</b>: Growth of <i>V. fischeri</i> in rich, undefined liquid medium</p><p><b>Alternate Protocol 1</b>: Growth of <i>V. fischeri</i> in minimal medium</p><p><b>Basic Protocol 3</b>: Storage of <i>V. fischeri</i> in frozen stocks</p><p><b>Basic Protocol 4</b>: Biofilm assay on solid agar</p><p><b>Alternate Protocol 2</b>: Biofilm assay in shaking liquid culture</p><p><b>Alternate Protocol 3</b>: Biofilm assay in static liquid culture</p><p><b>Basic Protocol 5</b>: Motility assay</p><p><b>Basic Protocol 6</b>: Luminescence assay</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":"57 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.103","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.103","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
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Abstract
Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri , as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri . © 2020 Wiley Periodicals LLC.
Basic Protocol 1 : Growth of V. fischeri from frozen stocks
Basic Protocol 2 : Growth of V. fischeri in rich, undefined liquid medium
Alternate Protocol 1 : Growth of V. fischeri in minimal medium
Basic Protocol 3 : Storage of V. fischeri in frozen stocks
Basic Protocol 4 : Biofilm assay on solid agar
Alternate Protocol 2 : Biofilm assay in shaking liquid culture
Alternate Protocol 3 : Biofilm assay in static liquid culture
Basic Protocol 5 : Motility assay
Basic Protocol 6 : Luminescence assay
费氏弧菌:实验室培养、储存和常见表型分析
费氏弧菌是一种与致病性弧菌相关的非致病性生物，可以很容易地在普通实验室设备中生长和储存。本文介绍了菲氏弧菌的常规生长、储存和表型评估方案，以及有用培养基的配方。具体来说，本文描述了该微生物在复杂和最小培养基中生长的程序和注意事项。它还描述了测定的生物膜形成，运动性和生物发光，三种常见的评估表型的费氏弧菌。©2020 Wiley期刊有限公司基本方案1:从冷冻储存库中生长的V. fischeri基本方案2:在丰富的，未定义的液体培养基中生长的V. fischeri备用方案1:在最小培养基中生长的V. fischeri基本方案3:在冷冻储存库中储存的V. fischeri基本方案4:在固体agon上的生物膜试验备用方案2:在晃动液体培养中进行生物膜试验备用方案3:在静态液体培养中进行生物膜试验基本方案5:活性试验基本方案6:发光分析
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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