An Overview on Mesenchymal Stem Cells Derived from Extraembryonic Tissues: Supplement Sources and Isolation Methods.

IF 1.7 Q4 CELL BIOLOGY Stem Cells and Cloning-Advances and Applications Pub Date : 2020-07-07 eCollection Date: 2020-01-01 DOI:10.2147/SCCAA.S248519

Parvin Salehinejad, Mojgan Moshrefi, Touba Eslaminejad

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引用次数: 8

Abstract

Purpose: The main aim of this review was to provide an updated comprehensive report regarding isolation methods of MSCs from human extra embryonic tissues, including cord blood, amniotic fluid, and different parts of the placenta and umbilical cord, with respect to the efficacy of these methods.

Results: Extra embryonic tissues are the most available source for harvesting of mesenchymal stem cells (MSCs). They make a large number of cells accessible using non-invasive methods of isolation and the least immune-rejection reactions. A successful culture of primary cells requires obtaining a maximum yield of functional and viable cells from the tissues. In addition, there are many reports associated with their differentiation into various kinds of cells, and there are some clinical trials regarding their utilization for patients.

Conclusion: Currently, cord blood-MSCs have been tested for cartilage and lung diseases. Umbilical cord-MSCs were tested for liver and neural disorders. However, these MSCs can be isolated, expanded, and cryopreserved in a cell bank for patients in need.

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胚胎外组织间充质干细胞研究综述:补充来源和分离方法。

目的:本综述的主要目的是提供一份关于从人类胚胎外组织(包括脐带血、羊水、胎盘和脐带的不同部分)中分离MSCs的方法的最新综合报告，以及这些方法的有效性。结果:胚胎外组织是获取间充质干细胞(MSCs)最有效的来源。它们使用非侵入性的分离方法和最小的免疫排斥反应使大量细胞可接近。原代细胞的成功培养需要从组织中获得最大产量的功能细胞和活细胞。此外，有许多报道与它们分化成各种细胞有关，也有一些关于它们在患者中的应用的临床试验。结论:目前，脐带血间充质干细胞已被用于软骨和肺部疾病的检测。脐带间充质干细胞检测肝脏和神经紊乱。然而，这些间充质干细胞可以分离、扩增，并在细胞库中冷冻保存，以供有需要的患者使用。

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