SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2020-12-01 DOI:10.1080/19336918.2020.1829264

Yusuke Mizutani, Daisuke Omagari, Manabu Hayatsu, Masaaki Nameta, Kazuo Komiyama, Yoshikazu Mikami, Tatsuo Ushiki

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引用次数: 3

Abstract

To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.

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SLPI通过调节板足/褶边和桥粒来促进细胞迁移，其中galectin - 4起重要作用。

为了阐明分泌性白细胞蛋白酶抑制剂(SLPI)诱导细胞迁移的潜在机制，我们使用多种显微成像方法和基因表达分析比较了SLPI缺失的人牙龈癌Ca9-22 (ΔSLPI)细胞和原始(野生型:wt) Ca9-22细胞。我们的结果表明，与wtCa9-22细胞相比，ΔSLPI细胞的迁移减少。与wtCa9-22细胞相比，ΔSLPI细胞的板足/背褶更小，移动更慢。此外，在ΔSLPI细胞的桥粒连接处观察到发育良好的中间丝束。此外，Galectin4在ΔSLPI细胞中强烈表达，其强制表达抑制wtCa9-22细胞的迁移。综上所述，SLPI通过调节板足/褶边和桥粒来促进细胞迁移，其中半乳糖凝集素4起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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