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{"title":"Purification of Yeast Spores to Investigate Their Dynamics of Activation","authors":"Samuel Plante, Christian R. Landry","doi":"10.1002/cpmc.123","DOIUrl":null,"url":null,"abstract":"<p>Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes the sporulation of wild <i>Saccharomyces cerevisiae</i> and <i>Saccharomyces paradoxus</i> strains, and the isolation and purification of ascospores. We also describe a method to synchronously induce germination in a spore population as well as to measure spore activation. This procedure can be applied, for example, to the study of environmental conditions that trigger germination. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Sporulation</p><p><b>Basic Protocol 2</b>: Spore purification</p><p><b>Basic Protocol 3</b>: Germination induction</p><p><b>Support Protocol 1</b>: Flow cytometry analysis</p><p><b>Support Protocol 2</b>: Heat-shock resistance measurement</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.123","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.123","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Germination is an important developmental process that supports resumption of growth in dormant spores. The study of the mechanisms underlying germination requires a pure spore population devoid of other cell types. This article describes the sporulation of wild Saccharomyces cerevisiae and Saccharomyces paradoxus strains, and the isolation and purification of ascospores. We also describe a method to synchronously induce germination in a spore population as well as to measure spore activation. This procedure can be applied, for example, to the study of environmental conditions that trigger germination. © 2020 Wiley Periodicals LLC.
Basic Protocol 1 : Sporulation
Basic Protocol 2 : Spore purification
Basic Protocol 3 : Germination induction
Support Protocol 1 : Flow cytometry analysis
Support Protocol 2 : Heat-shock resistance measurement
酵母孢子的纯化及其活化动力学研究
发芽是支持休眠孢子恢复生长的重要发育过程。对发芽机制的研究需要一个没有其他细胞类型的纯孢子群体。本文介绍了野生酿酒酵母菌和悖论酵母菌的产孢过程，以及子囊孢子的分离纯化。我们还描述了一种在孢子群体中同步诱导发芽以及测量孢子激活的方法。例如，这个程序可以应用于研究触发发芽的环境条件。©2020 Wiley期刊有限责任公司基本方案1:孢子萌发基本方案2:孢子纯化基本方案3:发芽诱导支持方案1:流式细胞术分析支持方案2:耐热冲击性测量
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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