Lanjie Yi, Yan Lu, Shun Yu, Qian Cheng, Lanjuan Yi
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引用次数: 13
Abstract
To investigate the effects of formononetin on rats with gastric ulcer and further to explore its possible mechanism. Rats were randomly divided into sham operation group (Sham), model group (Model), omeprazole control group (Omeprazole) and formononetin in different dose groups (FOR-L, FOR-M, FOR-H). Rats model with gastric ulcer were established by 100% glacial acetic acid. Hematoxylin-eosin (H&E) staining was used to observe the pathological morphology of gastric mucosa. Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the level of inflammatory and angiogenesis related factors. The expressions of nuclear factor kappa-B (NF-κB) signaling pathway-related proteins were detected by western blot. Formononetin and omeprazole could ameliorate the pathological morphology of gastric mucosa in gastric ulcer rats. Compared with Model group, the levels of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, IL-6, myeloperoxidase (MPO), human endothelin (ET)-1 and p-P65 protein in formononetin treatment and omeprazole groups were significantly decreased (p < 0.05). Moreover, formononetin could increase the content of vascular endothelial growth factor (VEGF), nitric oxide (NO) and the levels of CD34, tight junction proteins (ZO-1 and occludin) and p-IκBα in a dose-dependent manner. Formononetin can ameliorate gastric ulcer in rats by inhibiting inflammation and promoting gastric mucosal angiogenesis, and its mechanism maybe related to NF-κB signaling pathway.
目的观察芒柄花素对大鼠胃溃疡的影响,并进一步探讨其可能的作用机制。将大鼠随机分为假手术组(sham)、模型组(model)、奥美拉唑对照组(奥美拉唑)和刺芒柄花素不同剂量组(FOR-L、FOR-M、FOR-H)。采用100%冰醋酸建立大鼠胃溃疡模型。采用苏木精-伊红(H&E)染色法观察大鼠胃黏膜病理形态。采用免疫组织化学和酶联免疫吸附法(ELISA)检测炎症和血管生成相关因子的水平。western blot检测核因子κ b (NF-κB)信号通路相关蛋白的表达。刺芒柄花素和奥美拉唑能改善胃溃疡大鼠胃黏膜的病理形态。与模型组比较,刺芒柄花素组和奥美拉唑组大鼠肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6、髓过氧化物酶(MPO)、人内皮素(ET)-1和p- p65蛋白水平均显著降低(p
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