Pre-pregnancy exposure to fine particulate matter (PM2.5) increases reactive oxygen species production in oocytes and decrease litter size and weight in mice.
Yi Guo, Zhijuan Cao, Xianting Jiao, Dandan Bai, Yalin Zhang, Jing Hua, Wenqiang Liu, Xiaoming Teng
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引用次数: 17
Abstract
Exposure of females to fine particulate matter ≤2.5 μm in diameter (PM2.5) prior to pregnancy could produce adverse impact on fertility and enhances susceptibility of the offspring to a variety of diseases. In the current study, female C57BL/6 mice (6 weeks of age) were exposed to either concentrated PM2.5 or filtered air (average PM2.5 concentration: 115.60 ± 7.77 vs. 14.07 ± 0.38 μg/m-3) using a whole-body exposure device for 12 weeks. Briefly, PM2.5 exposure decreased anti-Müllerian hormone level (613.40 ± 17.36 vs 759.30 ± 21.90 pg mL-1, P<0.01) and increased reactive oxygen species (ROS) level (45.39 ± 0.82 vs 24.20 ± 0.85 arbitrary unit in fluorescence assay, P<0.01) in oocytes. The exposure increased oocyte degeneration rate (21.5% vs 5.1%, respectively (P<0.01) and decreased the 2-cell formation rate (71.9% vs 86.0%, P < 0.01). Transcriptome profiling using RNA sequencing showed wide spectrum of abnormal expression of genes, particularly those involved in regulating the mitochondrial respiratory complex in oocytes and metabolic processes in blastocysts. The exposure decreased litter size (6 ± 0.37 vs 7 ± 0.26, P<0.05) and weight (1.18 ± 0.02 vs 1.27 ± 0.02 g, P<0.01). In summary, PM2.5 exposure decreased female fertility, possibly through increased ROS production in oocytes and metabolic disturbances in developing embryos. The cause-effect relationship, however, requires further investigation.
雌性在怀孕前暴露于直径≤2.5 μm (PM2.5)的细颗粒物会对生育能力产生不利影响,并增加后代对多种疾病的易感。在本研究中,雌性C57BL/6小鼠(6周龄)使用全身暴露装置暴露于浓缩PM2.5或过滤空气(平均PM2.5浓度:115.60±7.77 vs 14.07±0.38 μg/m-3) 12周。PM2.5暴露降低卵母细胞抗勒氏激素水平(613.40±17.36 vs 759.30±21.90 pg mL-1, P<0.01),升高活性氧(ROS)水平(荧光测定45.39±0.82 vs 24.20±0.85任意单位,P<0.01)。暴露使卵母细胞变性率升高(21.5% vs 5.1%, P<0.01),使2细胞形成率降低(71.9% vs 86.0%, P<0.01)
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.