LncRNA SUMO1P3 regulates the invasion, migration and cell cycle of gastric cancer cells through Wnt/β-catenin signaling pathway.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Receptors and Signal Transduction Pub Date : 2021-12-01 Epub Date: 2020-11-12 DOI:10.1080/10799893.2020.1836494

Zhong Xu, Jing Ran, Kai Gong, Yihan Hou, Ji Li, Yijuan Guo

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引用次数: 5

Abstract

Objective: To investigate the regulatory effect of long non-coding RNA (lncRNA) SUMO1P3 on invasion, migration and cell cycle of gastric cancer (GC) cells through Wnt/β-catenin signaling pathway.

Methods: Tumor tissues and adjacent normal tissues from the GC patients were collected, and human normal gastric epithelial cells GES1 and GC cells SGC-7901, MKN45, HGC-27 and AGS were selected for study. The expression of SUMO1P3 in GC tissues and cells were detected by RT-qPCR. The effects of SUMO1P3 on the proliferation, invasion and migration of SGC-7901 and MKN45 cells were detected by CCK-8, transwell and wound healing assay respectively, and the effects of SUMO1P3 on apoptosis and cycle progression of SGC-7901 and MKN45 cells were detected by flow cytometry. The expressions of Wnt/β-catenin pathway-related and cell cycle-related proteins were detected by Western blot.

Results: The expression of SUMO1P3 was significantly upregulated in GC tissues and cell lines. Downregulation of SUMO1P3 significantly inhibited the SGC-7901 and MKN45 cell proliferation, invasion, migration, and cycle progression and promoted the cell apoptosis, while overexpression of SUMO1P3 showed the opposite effect. Further study showed that downregulation of SUMO1P3 significantly reduced the expressions of Wnt1, β-catenin, c-myc, and Cyclin D1 in SGC-7901 and MKN45 cells.

Conclusion: SUMO1P3 may promote invasion, migration, and cycle progression of SGC-7901 and MKN45 cells by enhancing the Wnt/β-catenin pathway.

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LncRNA SUMO1P3通过Wnt/β-catenin信号通路调控胃癌细胞的侵袭、迁移和细胞周期。

目的:探讨长链非编码RNA (lncRNA) SUMO1P3通过Wnt/β-catenin信号通路对胃癌(GC)细胞侵袭、迁移及细胞周期的调控作用。方法:收集胃癌患者的肿瘤组织及邻近正常组织，选取人正常胃上皮细胞GES1、胃癌细胞SGC-7901、MKN45、HGC-27、AGS进行研究。RT-qPCR检测胃癌组织和细胞中SUMO1P3的表达。采用CCK-8法、transwell法和创面愈合法分别检测SUMO1P3对SGC-7901和MKN45细胞增殖、侵袭和迁移的影响，采用流式细胞术检测SUMO1P3对SGC-7901和MKN45细胞凋亡和周期进展的影响。Western blot检测Wnt/β-catenin通路相关蛋白和细胞周期相关蛋白的表达。结果:在胃癌组织和细胞系中，SUMO1P3的表达明显上调。下调SUMO1P3可显著抑制SGC-7901和MKN45细胞的增殖、侵袭、迁移和周期进展，促进细胞凋亡，而过表达SUMO1P3则相反。进一步研究表明，下调SUMO1P3可显著降低SGC-7901和MKN45细胞中Wnt1、β-catenin、c-myc和Cyclin D1的表达。结论:SUMO1P3可能通过增强Wnt/β-catenin通路促进SGC-7901和MKN45细胞的侵袭、迁移和周期进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Receptors and Signal Transduction 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

>12 weeks

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