A cost effective SiO₂-CaO-Na₂O bio-glass derived from bio-waste resources for biomedical applications.

IF 4.4 3区医学 Q2 ENGINEERING, BIOMEDICAL Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-19 DOI:10.1007/s40204-020-00145-0

Srinath Palakurthy, K Venugopal Reddy, Sushil Patel, P Abdul Azeem

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引用次数: 16

Abstract

The present paper describes the in vitro bioactivity, cytocompatibility and degradation performance of SiO₂-CaO-Na₂O bio-glass synthesized using bio-waste. Egg shells and rice husk ash (RHA) bio-wastes were used as sources of calcium oxide (CaO) and silica (SiO₂), respectively. Glass samples were obtained by melt-quenching technique. Bioactivity was studied using in vitro experiments in simulated body fluid (SBF), degradation behaviour was evaluated in Tris-HCl buffer solutions recommended by ISO 10993-14 standards and cytocompatibility was estimated using MTT assay. The formation of hydroxyapatite was characterized by XRD, FTIR and SEM-EDS after soaking the glass samples in SBF solution. XRD confirmed the phase of hydroxyapatite with its standard JCPDS data. FTIR analyses revealed the occurrence of distinctive functional groups related to hydroxyapatite. Surface micrographs showed the agglomerated globular shape morphology of hydroxyapatite, while EDS analysis confirmed the existence of biological elements of apatite such as Ca, P and O. Degradation study results showed that the glass thus prepared has considerable controlled degradation rate. MTT assay revealed the cytocompatibility nature for different dosages (1000-50 μg/mL) of the prepared glass with MG-63 cells. These results perfectly established that egg shells and RHA are potentially beneficial resources for the production of bio-glasses.

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从生物废物资源中提取的具有成本效益的用于生物医学应用的SiO2-CaO-Na2O生物玻璃。

本文介绍了利用生物废弃物合成的二氧化硅- cao - na2o生物玻璃的体外生物活性、细胞相容性和降解性能。利用蛋壳和稻壳灰(RHA)生物废弃物分别作为氧化钙(CaO)和二氧化硅(SiO2)的来源。采用熔淬法制备玻璃样品。通过体外模拟体液(SBF)实验研究了其生物活性，在ISO 10993-14标准推荐的Tris-HCl缓冲溶液中评估了降解行为，并使用MTT法估计了细胞相容性。采用XRD、FTIR、SEM-EDS等方法对玻璃样品在SBF溶液中浸泡后羟基磷灰石的形成进行了表征。XRD用JCPDS标准数据证实了羟基磷灰石的物相。FTIR分析显示与羟基磷灰石相关的特殊官能团的出现。表面显微照片显示羟基磷灰石呈球状凝聚形态，能谱分析证实了磷灰石中Ca、P、o等生物元素的存在。降解研究结果表明，由此制备的玻璃具有相当的可控降解率。MTT实验显示不同剂量(1000 ~ 50 μg/mL)制备的玻璃与MG-63细胞的相容性。这些结果完美地证明了蛋壳和RHA是生产生物玻璃的潜在有益资源。

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来源期刊

Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

9.60

自引率

4.10%

发文量

期刊介绍： Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.