Characterization of the Gene Expression Patterns in the Murine Liver Following Intramuscular Administration of Baculovirus.

Q2 Biochemistry, Genetics and Molecular Biology Gene expression Pub Date : 2021-06-11 Epub Date: 2020-10-28 DOI:10.3727/105221620X16039045978676

Mitsuhiro Iyori, Ryohei Ogawa, Talha Bin Emran, Shuta Tanbo, Shigeto Yoshida

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Abstract

Intramuscular administration of wild-type baculovirus is able to both protect against Plasmodium sporozoite challenge and eliminate liver-stage parasites via a Toll-like receptor 9-independent pathway. To investigate its effector mechanism(s), the gene expression profile in the liver of baculovirus-administered mice was characterized by cDNA microarray analysis. The ingenuity pathway analysis gene ontology module revealed that the major gene subsets induced by baculovirus were immune-related signaling, such as interferon signaling. A total of 40 genes commonly upregulated in a Toll-like receptor 9-independent manner were included as possible candidates for parasite elimination. This gene subset consisted of NT5C3, LOC105246895, BTC, APOL9a/b, G3BP3, SLC6A6, USP25, TRIM14, and PSMB8 as the top 10 candidates according to the special unit. These findings provide new insight into effector molecules responsible for liver-stage parasite killing and, possibly, the development of a new baculovirus-mediated prophylactic and therapeutic biopharmaceutical for malaria.

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小鼠肌肉注射杆状病毒后肝脏基因表达模式的表征。

肌内注射野生型杆状病毒能够通过toll样受体9不依赖的途径抵御疟原虫孢子虫的攻击并消除肝期寄生虫。为了研究其作用机制，采用cDNA芯片分析方法对杆状病毒小鼠肝脏中的基因表达谱进行了表征。独创性途径分析基因本体模块显示杆状病毒诱导的主要基因亚群是免疫相关信号，如干扰素信号。共有40个基因通常以toll样受体9独立的方式上调，作为寄生虫消除的可能候选基因。该基因亚群由NT5C3、LOC105246895、BTC、APOL9a/b、G3BP3、SLC6A6、USP25、TRIM14和PSMB8作为按特殊单元排列的前10个候选基因组成。这些发现提供了对负责肝期寄生虫杀伤的效应分子的新见解，并可能开发出一种新的杆状病毒介导的疟疾预防和治疗生物制药。

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来源期刊

Gene expression 生物-生物工程与应用微生物

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Gene Expression, The Journal of Liver Research will publish articles in all aspects of hepatology. Hepatology, as a research discipline, has seen unprecedented growth especially in the cellular and molecular mechanisms of hepatic health and disease, which continues to have a major impact on understanding liver development, stem cells, carcinogenesis, tissue engineering, injury, repair, regeneration, immunology, metabolism, fibrosis, and transplantation. Continued research and improved understanding in these areas will have a meaningful impact on liver disease prevention, diagnosis, and treatment. The existing journal Gene Expression has expanded its focus to become Gene Expression, The Journal of Liver Research to meet this growing demand. In its revised and expanded scope, the journal will publish high-impact original articles, reviews, short but complete articles, and special articles (editorials, commentaries, opinions) on all aspects of hepatology, making it a unique and invaluable resource for readers interested in this field. The expanded team, led by an Editor-in-Chief who is uniquely qualified and a renowned expert, along with a dynamic and functional editorial board, is determined to make this a premier journal in the field of hepatology.