Frequency of IL-10+CD19+ B cells in patients with prostate cancer compared to patients with benign prostatic hyperplasia.

IF 0.9 4区医学 Q3 MEDICINE, GENERAL & INTERNAL African Health Sciences Pub Date : 2020-09-01 DOI:10.4314/ahs.v20i3.31

Nazmabadi Roya, Taheri Fatemeh, Mohammad-Alibeigi Faramarz, Sabzevary-Ghahfarokhi Milad, Sanaei Mohammad-Javad, Salehi-Vanani Najmeh, Mirzaei Yousef, Bagheri Nader

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引用次数: 14

Abstract

Background: The function of the immune system in prostate cancer (PC) might promote carcinogenesis. PC is a common cancer in men. Regulatory B cells (Bregs) are a new subtype of B cells that have suppressive roles in the immune system. Interleukin-10 (IL-10) is a dominant mediator of immune suppression released by Bregs.

Objective: The purpose of this research was to examine the frequency of CD19+IL10+ B cells and IL-10 mRNA expression in patients with PC compared to patients with benign prostatic hyperplasia (BPH).

Methods: Forty paraffin tissue samples from patients with PC and 32 paraffin tissue samples from patients with BPH were entered in this study. The immunohistochemistry staining was used to evaluate the pattern expression of CD19 and IL-10 markers. IL-10 mRNA expression in fresh tissue was determined by real time-polymerase chain reaction (RT-PCR).

Results: The frequency of CD19+IL-10+ B cells and IL-10 mRNA expression in PC patients were significantly higher than patients with BPH. Also, there was no meaningful relationship between the frequency of IL-10+CD19+ B cells and gleason scores in patients with PC.

Conclusions: Our findings suggested that frequency of IL-10+CD19+ B cells correlates with progressive stage of PC.

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前列腺癌患者与良性前列腺增生患者IL-10+CD19+ B细胞频率的比较

背景:免疫系统在前列腺癌(PC)中的作用可能促进癌变。PC是男性常见的癌症。调节性B细胞(Regulatory B cells, Bregs)是一种新的B细胞亚型，在免疫系统中具有抑制作用。白细胞介素-10 (IL-10)是Bregs释放的免疫抑制的主要介质。目的:比较前列腺癌(PC)患者与良性前列腺增生(BPH)患者CD19+IL10+ B细胞频率及IL-10 mRNA表达的差异。方法:选取40例PC患者石蜡组织标本和32例BPH患者石蜡组织标本。免疫组化染色评价CD19和IL-10标志物的表达模式。采用实时聚合酶链反应(RT-PCR)检测新鲜组织中IL-10 mRNA的表达。结果:PC患者CD19+IL-10+ B细胞频率及IL-10 mRNA表达明显高于BPH患者。此外，PC患者IL-10+CD19+ B细胞的频率与gleason评分之间无显著关系。结论:我们的研究结果提示IL-10+CD19+ B细胞的频率与PC的进展阶段相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

African Health Sciences MEDICINE, GENERAL & INTERNAL-

CiteScore

2.30

自引率

0.00%

发文量

179

审稿时长

>12 weeks

期刊介绍： The African Health Sciences is an internationally refereed journal publishing original articles on research, clinical practice, public health, policy, planning, implementation and evaluation, in the health and related sciences relevant to Africa and the tropics. Its objectives are to: Advocate for and promote the growth of reading culture in sub Saharan Africa; Provide a high quality journal in which health and policy and other researchers and practitioners in the region can and world wide, can publish their work; Promote relevant health system research and publication in the region including alternative means of health care financing, the burden of and solution of health problems in marginalized urban and rural communities amongst the displaced and others affected by conflict; Promote research and the systematic collection and collation and publication of data on diseases and conditions of equity and influence; Promote development of evidence-based policies and guidelines for clinical, public health and other practitioners. African Health Sciences acknowledges support provided by the African Health Journals Partnership Project that is funded by the US National Institutes of Health (through the National Library of Medicine and the Fogarty International Center) and facilitated by the Council of Science Editors.