Antifungal activity of amniotic fluid against different Candida species

IF 1.5 4区生物学 Q4 MYCOLOGY Revista Iberoamericana De Micologia Pub Date : 2021-07-01 DOI:10.1016/j.riam.2021.02.002

Sofia Maraki , Dimitra Stafylaki , Spyridon A. Karageorgos , Filippos Koutroumpakis , George Hamilos , Antonis Makrigiannakis , George Samonis

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Abstract

Background

Although Candida is a commensal of the urogenital tract, intrauterine fungal infections are extremely uncommon in clinical practice.

Aims

In the present work we evaluated whether amniotic fluid (AF) possesses direct antifungal activity against clinical isolates of Candida albicans and other Candida species.

Methods

A total of 23 AF samples from pregnant women with gestational age of 38–41 weeks were obtained under aseptic conditions by the aspiration of the amniotic sac during cesarean section. Different Candida species were inoculated in amniotic fluid and Sabouraud broth, used as control, and were incubated at 37 °C for 48 h. Quantitative cultures of test samples and controls were performed at 0, 4, 8, 12, 24, and 48 h.

Results

AF collected from 23 pregnant women had consistent and significant inhibitory activity against all Candida isolates tested. Nonetheless, a complete inhibition of growth by all 23 AF samples tested was observed only against Candida glabrata.

Conclusions

It is likely that the antifungal activity of the AF against C. albicans, C. glabrata and Candida parapsilosis observed in vitro also exists in vivo, contributing to protect against intrauterine fungal infections.

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羊水对不同念珠菌的抑菌活性研究

背景:虽然念珠菌是泌尿生殖道的共生体，但宫内真菌感染在临床实践中极为罕见。目的评价羊水对临床分离的白色念珠菌和其他念珠菌是否具有直接的抗真菌活性。方法采用无菌条件下剖宫产术中羊膜囊抽吸法采集胎龄38 ~ 41周孕妇房颤样本23例。分别在羊水和Sabouraud肉汁中接种不同种类的念珠菌作为对照，37℃孵育48 h，分别在0、4、8、12、24和48 h进行定量培养。结果从23例孕妇中采集的saf对所有念珠菌均具有一致且显著的抑制活性。尽管如此，所有23个AF样品的生长完全抑制仅观察到对光滑假丝酵母的抑制作用。结论AF对体外观察到的白色念珠菌、光秃念珠菌和假丝酵母的抗真菌活性可能在体内也存在，对宫内真菌感染具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.