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Pneumocystis jirovecii in the lower respiratory tract of immunocompetent individuals.
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2025-01-25 DOI: 10.1016/j.riam.2024.10.002
Beatriz Gálvez, Consuelo Ferrer, Violeta Esteban, José Noberto Sancho-Chust, Beatriz Amat, Eusebi Chiner, Maria Francisca Colom

Background: Pneumocystis jirovecii colonization rates in healthy patients are unclear. Previously published studies suggest that the fungus could play a role in the physiopathology and progression of chronic respiratory diseases.

Aims: The goal of this study was to determine the prevalence of colonization by this fungus in the lower respiratory tract of immunocompetent patients who are not at risk of dysbiosis.

Methods: The presence of P. jirovecii was confirmed in the bronchoalveolar lavage (BAL) samples from adults who underwent bronchoscopy for non-infectious reasons, had no immunosuppressive factors, and had not been on antibiotic treatment for at least one month. The results were compared with those obtained in the study on the presence of Pneumocystis in environmental dust samples obtained by swabbing surfaces in the participating subjects' domestic settings. Real-time PCR was the technique used for detecting the fungus in both types of samples.

Results: A total of 97 BAL samples and 49 domestic environment samples were studied. The medical reasons for needing a bronchoscopy were, mainly, the examination of both pulmonary neoplasm in 55 patients (57%) and diffuse interstitial lung disease in 21 patients (22%). The overall prevalence of P. jirovecii in our population was 7.22% in BAL samples and 0% in domestic samples.

Conclusions: The presence of P. jirovecii in the lower respiratory tract is relevantly linked with the patient's immune status, not with an underlying pathology. Prevalence is low in immunocompetent individuals who do not have any infectious pathology and are not having antimicrobial treatments. Our results do not enable us to figure out which the environmental niche of P. jirovecii is.

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引用次数: 0
Fungal ball due to a species of the Scedosporium apiospermum complex in a post-tuberculosis patient 结核后患者的一种顶孢复合体引起的真菌球。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-04-01 DOI: 10.1016/j.riam.2024.07.001
Gabriela López Daneri , Natali Niebles , Agustina Bustos , Pablo Martin Bravo , Edith Susana Paterno , Fernando Picco , Alejandra Margari , María Teresa Mujica

Background

Scedosporium species are considered emerging pathogens causing illness in immunocompetent and immunocompromised hosts.

Case report

A case of non-invasive pulmonary (fungal ball) infection by Scedosporium apiospermum complex in a 49-year-old female with bronchiectasis and cavities secondary to tuberculosis is described. The patient had a history of three years of cough and hemoptysis. A computed tomography scan of the thorax revealed the presence of a cavity in the lower lobe of the right lung, associated with bronchiectasis. A combination of surgical debridement and antifungal therapy (voriconazole) was the treatment of choice. Pulmonary resection (right lower lobectomy) was performed, and samples were sent for microbiological culture and histopathological examination; by means of the latter technique, hyphae were shown. The identification of Scedosporium angustum, a phylogenetic species of the S. apiospermum complex, was obtained by amplifying and sequencing the β-tubulin locus. Voriconazole therapy was started at a loading dose of 800 mg/12 h for the first 24 h, followed by 200 mg/12 h for 6 months. The patient responded favorably to the treatment and remained asymptomatic.

Conclusions

This case emphasizes the importance of considering Scedosporium species in the differential diagnosis of fungal balls by Aspergillus.
.
背景:隐孢子菌被认为是在免疫功能正常和免疫功能低下的宿主中引起疾病的新兴病原体。病例报告:一个非侵入性肺(真菌球)感染的病例顶精子梭孢复合体在49岁的女性支气管扩张和继发于肺结核腔。患者有三年咳嗽咯血病史。胸部电脑断层扫描显示右肺下叶有空洞,伴支气管扩张。手术清创联合抗真菌治疗(伏立康唑)是首选的治疗方法。行肺切除术(右下肺叶切除术),送标本进行微生物培养和组织病理学检查;通过后一种技术,菌丝被显示出来。通过扩增β-微管蛋白位点并对其序列进行测序,确定了尖孢霉复合体的一个系统发育物种——Scedosporium angustum。伏立康唑治疗开始时的负荷剂量为800mg/12h,前24h,随后为200mg/12h,持续6个月。病人对治疗反应良好,并保持无症状。结论:本病例强调了在曲霉真菌球的鉴别诊断中考虑孢子菌种类的重要性。
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引用次数: 0
Nails and skin co-infection by Fusarium verticillioides and Proteus vulgaris secondary to arterial occlusion of lower extremity 下肢动脉闭塞继发的黄萎病镰刀菌和寻常变形杆菌并发指甲和皮肤感染。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-04-01 DOI: 10.1016/j.riam.2021.07.003
Yahui Feng , Zhiya Yang , Dengli Li , Juanjuan Li , Dongmei Li , Sybren de Hoog , Dongmei Shi

Background

Post-traumatic Fusarium infection is rare. Arterial occlusive disease, a common vascular disorder in the elderly, often leads to ischemic necrosis of the lower extremities, which in turn increases the likelihood of secondary infections. Those secondary infections can be caused by bacteria, virus, or fungi.

Case report

We present the case of a 64-year-old male patient with a co-infection by Fusarium verticillioides and Proteus vulgaris on nails and foot skin, secondary to senile arterial occlusion on the lower extremities. The skin and nails recovered well after following stent implantation and a combination treatment of itraconazole and a macrolide antibiotic. A retrospective analysis of the literature identified 17 patients with Fusarium infection, all of whom were immunocompetent.

Conclusions

Trauma may be a predisposing cause of Fusarium infection. Combination of oral itraconazole and terbinafine, or amphotericin B and surgical means, are all effective treatments.
背景:创伤后镰刀菌感染是罕见的。动脉闭塞性疾病是老年人常见的血管疾病,常导致下肢缺血性坏死,进而增加继发感染的可能性。这些继发性感染可能由细菌、病毒或真菌引起。病例报告:我们报告一例64岁男性患者,指甲和足部皮肤同时感染黄斑镰刀菌和寻常变形杆菌,继发于下肢老年性动脉闭塞。皮肤和指甲在支架植入和伊曲康唑和大环内酯类抗生素联合治疗后恢复良好。回顾性分析文献发现17例镰孢菌感染患者,所有患者均具有免疫功能。结论:外伤可能是镰刀菌感染的易感原因。口服伊曲康唑联合特比萘芬或两性霉素B联合手术治疗均是有效的治疗方法。
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引用次数: 0
Aspergillus hiratsukae as a causative agent of endophthalmitis: A case report 作为眼内炎致病菌的平曲霉:病例报告。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-04-01 DOI: 10.1016/j.riam.2024.10.001
Cristian Mateo León , Ángeles Sampere Martínez , Isabel Fabelo Hidalgo , Diego García Martínez de Artola

Background

Aspergillus flavus and Aspergillus fumigatus are commonly involved in fungal endophthalmitis. However, other less pathogenic species of the genus are also causal agents of opportunistic infections. Aspergillus hiratsukae, a species within Aspergillus section Fumigati, is an uncommon fungus responsible for a few infectious cases worldwide.

Case report

We present a case of post-surgical endophthalmitis in a man caused by A. hiratsukae after phacoemulsification surgery and intraocular lens implantation. The patient underwent an urgent vitrectomy in which infectious foci were observed. Due to adverse clinical evolution, evisceration was performed five days after admission. In the study of the intraoperative samples fungal growth was observed. Microscopic analysis showed conidial heads resembling Aspergillus species. The final identification of A. hiratsukae was achieved by amplifying and sequencing the ITS1-5.8S-ITS2 spacer region. Antifungal susceptibility testing was performed using the method described by EUCAST. The isolate exhibited sensitivity to azoles and polyenes.

Conclusions

Due to the morphological similarity of the Aspergillus species, molecular techniques are essential for discriminating among them, as there are different antifungal susceptibility profiles within the same complex. Further studies to improve clinical surveillance, make an early diagnosis and start a targeted therapy are necessary.
背景:黄曲霉和烟曲霉通常与真菌性眼内炎有关。然而,其他致病性较低的属也是机会性感染的致病因子。平松曲霉是烟曲霉科的一种,是一种罕见的真菌,在世界范围内引起一些传染性病例。病例报告:我们报告一例在超声乳化手术和人工晶状体植入术后,由平突芽胞杆菌引起的术后眼内炎。患者接受了紧急玻璃体切除术,其中观察到感染灶。由于临床进展不良,在入院5天后进行了内脏切除。术中标本观察真菌生长情况。显微分析显示分生孢子头类似曲霉属。通过对ITS1-5.8S-ITS2间隔区进行扩增和测序,最终鉴定了平松麻属。采用EUCAST方法进行抗真菌药敏试验。该分离物对偶氮和多烯敏感。结论:由于曲霉种类的形态相似性,在同一复合体内存在不同的抗真菌药敏谱,因此分子技术是鉴别曲霉种类的必要手段。进一步研究以改善临床监测、早期诊断和开始靶向治疗是必要的。
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引用次数: 0
Prevalence, virulence factors and antifungal susceptibility of oral isolates of Candida albicans from patients with cystic fibrosis in Mexico 墨西哥囊性纤维化患者口腔白色念珠菌的流行、毒力因素和抗真菌敏感性
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-04-01 DOI: 10.1016/j.riam.2024.09.001
Ana L. Ríos-López, María Fernanda Garza-Velásquez, Gloria M. González, Miguel A. Becerril-García, Orlando Flores-Maldonado

Background

Candida species are frequently isolated from the oral cavity of patients with cystic fibrosis. However, the information on the role of Candida in cystic fibrosis is scarce.

Aims

This study aimed to evaluate the prevalence, virulence profile and antifungal susceptibility of oral isolates of Candida albicans recovered from patients with cystic fibrosis.

Methods

Oropharyngeal swab samples were collected from sixty-five cystic fibrosis patients and sixty-five healthy individuals. Candida isolates were identified by MALDI-TOF VITEK-MS. Proteinase, phospholipase and esterase activity, biofilm production and level expression of ALS, SAP and PLB genes in C. albicans were evaluated. Minimal inhibitory concentration values were determined by means of an antifungal susceptibility test.

Results

Oral Candida colonization in cystic fibrosis patients was 66.15%, while in healthy individuals was 36.92%. C. albicans was the most frequently isolated species. C. albicans strains from cystic fibrosis patients were high producers of protease and biofilm, and had higher expression levels of adhesin and protease-associated genes in comparison with healthy subjects. Among the C. albicans strains isolated from cystic fibrosis patients, 18.91% were resistant to itraconazole, while 16.21% exhibited resistance to ketoconazole and fluconazole, and only one strain was resistant to voriconazole.

Conclusions

This work represents a surveillance study on virulence patterns and antifungal susceptibility of Candida from the oropharyngeal tract in cystic fibrosis.
背景:念珠菌经常从囊性纤维化患者的口腔中分离出来。然而,关于念珠菌在囊性纤维化中的作用的信息很少。目的:本研究旨在评估囊性纤维化患者口腔分离的白色念珠菌的患病率、毒力谱和抗真菌敏感性。方法:采集65例囊性纤维化患者和65例健康人的口咽拭子标本。采用MALDI-TOF VITEK-MS对念珠菌分离株进行鉴定。研究了白念珠菌的蛋白酶、磷脂酶和酯酶活性、生物膜的生成以及ALS、SAP和PLB基因的表达水平。通过抗真菌药敏试验确定最小抑制浓度值。结果:囊性纤维化患者口腔念珠菌定植率为66.15%,健康人群为36.92%。白色念珠菌是最常见的分离种。来自囊性纤维化患者的白色念珠菌菌株是蛋白酶和生物膜的高生产者,与健康受试者相比,粘连素和蛋白酶相关基因的表达水平更高。从囊性纤维化患者分离的白色念珠菌中,18.91%对伊曲康唑耐药,16.21%对酮康唑和氟康唑耐药,伏立康唑耐药的仅有1株。结论:这项工作代表了囊性纤维化口咽道念珠菌毒力模式和抗真菌敏感性的监测研究。
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引用次数: 0
Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response 卡氏肺囊虫感染导致 Fn1 表达上调,从而引起肺纤维化和炎症反应。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.riam.2024.04.002
Wenwen Yu , Hua Ye , Yunlei Li , Xiaoqiong Bao, Yangyang Ni, Xiangxiang Chen, Yangjie Sun, Ali Chen, Weilong Zhou, Jifa Li

Background

Pneumocystis carinii is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.

Aims

This study attempted to investigate the role of P. carinii infection-related genes in regulating lung fibrosis in mice.

Methods

A screening of P. carinii infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain P. carinii infection-related key genes. The development of a mouse model with gene aberrant expression was achieved by utilizing mice carrying the Cre-LoxP recombinase system. Dexamethasone was employed to induce tracheal infection in order to develop a model of pulmonary fibrosis, and the magnitude of lung injury was assessed by performing hematoxylin–eosin (H&E) staining and Masson staining. Lung coefficient and hydroxyproline level were assessed on sections of lung tissue as well. Finally, the magnitude of lung fibrosis and inflammation in mice was determined based on immunofluorescence and on the expression of genes associated with lung fibrosis and inflammation.

Results

Fn1 was found by PPI with the highest connectivity in the PPI network associated with immunity and inflammation. Besides, Fn1 was significantly highly expressed in P. carinii-infected mice samples. The P carinii pneumonia (PCP)+Fn1fl/fl group had significantly higher lung coefficients, hydroxyproline levels and TNF-α, IL-6, IL-1β, IL-8 and NLRP3 expression levels, and significantly lower IL-10 expression levels. The results found in PCP+SPC-Cre:Fn1fl/fl group were the opposite. The results of the pulmonary fibrosis level study showed that the PCP+Fn1fl/fl group had the most intense H&E and Masson staining, and significantly higher expression levels of Col1A2, Col3A1 and α-SMA, which were lower in the PCP+SPC-Cre:Fn1fl/fl group.

Conclusions

P. carinii infection may promote the upregulation of Fn1, which causes pulmonary fibrosis with an inflammatory response.
背景:目的:本研究试图探讨卡氏肺囊虫感染相关基因在调控小鼠肺纤维化中的作用:方法:利用GEO数据库对卡氏痢疾杆菌感染相关的差异mRNA进行筛选,然后利用STRING网站构建蛋白-蛋白相互作用(PPI)网络,以获得卡氏痢疾杆菌感染相关的关键基因。利用携带 Cre-LoxP 重组酶系统的小鼠建立了基因异常表达的小鼠模型。利用地塞米松诱导气管感染以建立肺纤维化模型,并通过苏木精-伊红(H&E)染色和马森染色评估肺损伤的程度。此外,还对肺组织切片的肺系数和羟脯氨酸水平进行了评估。最后,根据免疫荧光以及肺纤维化和炎症相关基因的表达确定了小鼠肺纤维化和炎症的程度:结果:PPI发现,在与免疫和炎症相关的PPI网络中,Fn1的连接性最高。此外,Fn1在卡里茵肺炎感染小鼠样本中明显高表达。卡里茵肺炎(PCP)+Fn1fl/fl组的肺系数、羟脯氨酸水平、TNF-α、IL-6、IL-1β、IL-8和NLRP3表达水平明显升高,而IL-10表达水平明显降低。PCP+SPC-Cre:Fn1fl/fl组的结果则相反。肺纤维化水平研究结果显示,PCP+Fn1fl/fl组的H&E和Masson染色最浓,Col1A2、Col3A1和α-SMA的表达水平明显较高,而PCP+SPC-Cre:Fn1fl/fl组则较低:结论:卡氏杆菌感染可能会促进Fn1的上调,从而导致肺纤维化并伴有炎症反应。
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引用次数: 0
Clinical response to prednisone in a severe case of infant-juvenile paracoccidioidomycosis 一例严重的婴幼儿副球孢子菌病患者对泼尼松的临床反应。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.riam.2024.04.001

Background

Paracoccidioidomycosis is a neglected tropical disease caused by fungi of the genus Paracoccidioides. A wide range of symptoms is related to the disease; however, lungs and skin are the sites predominantly affected. The disease is mostly seen in people living in rural areas in Latin America.

Case report

We present a pediatric case of severe disseminated paracoccidioidomycosis that slowly responded to the antifungal treatment. Within three months, symptoms evolved into hepatosplenomegaly, necrotic cervical and abdominal lymph nodes, and splenic abscess. Clinical response to amphotericin B deoxycholate and itraconazole was slow, resulting in pleural and peritoneal cavity effusions, heart failure and shock. Amphotericin B deoxycholate was replaced by the liposomal formulation, with no response. Subsequently, prednisone was added to the treatment, which led to improvement in the clinical response. Serological Paracoccidioides antibody titers were atypical, with very low titers in the critical phase and significant increase during the convalescence phase. The infection was finally cleared up with amphotericin B deoxycholate, liposomal amphotericin B and the use of corticosteroids. Paracoccidioidomycosis serology was non-reactive two years post-discharge.

Conclusions

Due to the intense inflammatory response triggered by Paracoccidioides cells, giving low-dose prednisone for a short period of time modulated the inflammatory response and supported antifungal treatment.
背景:副球孢子菌病是由副球孢子菌属真菌引起的一种被忽视的热带疾病。该病的症状多种多样,但主要受累部位是肺部和皮肤。这种疾病主要发生在拉丁美洲的农村地区:我们报告了一例严重的播散性副球孢子菌病儿科病例,患者对抗真菌治疗反应缓慢。三个月内,症状演变为肝脾肿大、颈部和腹部淋巴结坏死以及脾脓肿。脱氧胆酸两性霉素 B 和伊曲康唑的临床反应缓慢,导致胸腔和腹腔积液、心力衰竭和休克。脱氧胆酸两性霉素 B 被脂质体制剂取代,但无反应。随后,在治疗中加入了泼尼松,临床反应有所改善。血清学中的副球孢子菌抗体滴度不典型,在临界期滴度很低,而在恢复期滴度显著升高。通过使用脱氧胆酸两性霉素 B、两性霉素 B 脂质体和皮质类固醇,感染最终被清除。出院后两年,副球孢子菌病血清学检查未见反应:结论:由于副球孢子菌细胞引发了强烈的炎症反应,短期使用小剂量泼尼松可调节炎症反应,支持抗真菌治疗。
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引用次数: 0
Fungemia due to opportunistic fungi in patients with cancer 癌症患者因机会性真菌引起的菌血症。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.riam.2024.01.001

Background

Fungemia due to uncommon fungi and secondary to multiple risk factors has become an emergent health problem, particularly in oncology patients.

Aims

This study shows the following data collected during an 11-year period in a tertiary care oncologic center from patients with fungemia: demographic data, clinical characteristics, and outcome.

Methods

A retrospective study was performed at Instituto Nacional de Cancerología, a 135-bed referral cancer center in Mexico City, from July 2012 to June 2023. All episodes of non-Candida fungemia were included.

Results

Sixteen cases with uncommon fungemia were found in the database, representing 0.3% from all the blood cultures positive during the study period, and 8.5% from all the fungi isolated. The most common pathogens identified in our series were Histoplasma capsulatum, Acremonium spp., Trichosporon asahii, and Saccharomyces cerevisiae. Eight patients had hematologic malignancies, and five had severe neutropenia. In eight cases fungemia was considered catheter-related, in four cases was classified as primary, and in the last four it was diagnosed as disseminated fungal diseases. Mortality at 30 days was 43.8%.

Conclusions

The improved diagnostic tools have led to a better diagnosis of uncommon fungal infections. More aggressive therapeutic approaches, particularly in patients with malignancies, would increase survival rates in these potentially fatal diseases.
背景:由不常见真菌引起并继发于多种风险因素的真菌血症已成为一个紧迫的健康问题,尤其是在肿瘤患者中。目的:本研究显示了在一家三级肿瘤中心的11年间收集的真菌血症患者的以下数据:人口统计学数据、临床特征和结果:2012年7月至2023年6月,在墨西哥城一家拥有135张床位的癌症转诊中心--国立癌症研究所(Instituto Nacional de Cancerología)进行了一项回顾性研究。研究纳入了所有非念珠菌性真菌病病例:结果:数据库中发现了16例非典型真菌血症病例,占研究期间所有血液培养阳性病例的0.3%,占所有真菌分离病例的8.5%。在我们的系列研究中,最常见的病原体是荚膜组织胞浆菌(Histoplasma capsulatum)、Acremonium spp.、旭三孢子菌(Trichosporon asahii)和酿酒酵母菌(Saccharomyces cerevisiae)。八名患者患有血液系统恶性肿瘤,五名患者患有严重的中性粒细胞减少症。8例真菌血症被认为与导管有关,4例被归类为原发性,最后4例被诊断为播散性真菌病。30天后的死亡率为43.8%:结论:诊断工具的改进提高了对不常见真菌感染的诊断率。更积极的治疗方法,尤其是针对恶性肿瘤患者的治疗方法,将提高这些潜在致命疾病的存活率。
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引用次数: 0
Azole resistance in a clinical isolate of Aspergillus fumigatus from Chile 智利烟曲霉临床分离株的唑类抗药性。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.riam.2024.04.003
Eduardo Álvarez Duarte , Nicolás Cepeda , Jean Miranda

Background

Aspergillus fumigatus is a ubiquitous opportunistic pathogen. This fungus can acquire resistance to azole antifungals due to different mutations in the cyp51A gene. Azole resistance has been observed in several continents and appears to be a globally distributed phenomenon. Specific mutations in cyp51A that lead to azole resistance, such as the TR34/L98H modification, have been reported.

Aims

To evaluate the azole resistance in clinically isolated A. fumigatus strains.

Methods

As a result of our passive surveillance strategy, a total of 23 A. fumigatus isolates from clinical origins were identified through a phylogenetic analysis using the ITS region and β-tubulin gene fragments, and typed with the CSP microsatellite. Azole susceptibility profiles were performed by disk diffusion and microdilution broth methodologies according to CLSI guidelines.

Results

Here we describe, for the first time, the detection of azole-resistant A. fumigatus isolates from clinical origins in Chile with mutations in the cyp51A gene. In addition to the TR34/L98H mutation, one isolate exhibited an F46Y/M172V/E427K-type mutation. Furthermore, microsatellite typing based on cell surface protein (CSP) was performed, showing the t02 (TR34/L98H), t15 (F46Y/M172V/E427K) and t01 (susceptible clinical isolates) genotypes.

Conclusions

Our study demonstrates the presence of mutations related to azole resistance in A. fumigatus strains isolated from clinical samples in Chile. In order to obtain information that may help to tackle the spread of antifungal resistance among A. fumigatus populations, and to ensure the efficacy of future treatments against aspergillosis, a further research is necessary.
背景:烟曲霉是一种无处不在的机会性病原体。由于 cyp51A 基因的不同突变,这种真菌可获得对唑类抗真菌药的抗药性。唑类抗真菌剂的抗药性已在几大洲被观察到,而且似乎是一种全球分布现象。目的:评估临床分离的烟曲霉菌株的唑类抗药性:方法:通过被动监测策略,利用 ITS 区域和 β-管蛋白基因片段进行系统发育分析,确定了 23 株临床分离的烟曲霉菌株,并用 CSP 微卫星进行了分型。根据 CLSI 指南,通过盘式扩散和微量稀释肉汤法进行了唑类药敏性分析:结果:我们在此首次发现了来自智利临床的耐唑烟曲霉分离物,其cyp51A基因发生了突变。除了 TR34/L98H 突变外,其中一个分离株还出现了 F46Y/M172V/E427K 型突变。此外,还进行了基于细胞表面蛋白(CSP)的微卫星分型,显示出 t02(TR34/L98H)、t15(F46Y/M172V/E427K)和 t01(易感临床分离株)基因型:我们的研究表明,从智利临床样本中分离出的烟曲霉菌株中存在与唑类抗性相关的突变。为了获得有助于解决烟曲霉菌抗药性扩散问题的信息,并确保未来治疗曲霉菌病的疗效,有必要开展进一步的研究。
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引用次数: 0
Experimental study of specific and nonspecific blood culture bottles for the diagnosis of candidemia 用于诊断念珠菌血症的特异性和非特异性血液培养瓶的实验研究。
IF 1.5 4区 生物学 Q4 MYCOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.riam.2024.06.001
Leandre Carmem Wilot , Vanice Rodrigues Poester , Cecília Bittencourt Severo , Karine Ortiz Sanchotene , Bruna Muradás Esperon , Mariana Rodrigues Trápaga , David A. Stevens , Melissa Orzechowski Xavier

Background

Early diagnosis of candidemia is critical for the correct management and treatment of patients.

Aims

To test the efficacy of different blood culture bottles in the growth of Candida strains.

Methods

We compared the performance of BD BACTEC™ Plus Aerobic/F (Aero) culture bottles with the specific BD BACTEC™ Mycosis IC/F Lytic (Myco) culture bottles using the BD BACTEC™ FX 40 automated blood culture system to determine the mean time-to-detection (TTD) in Candida species. One isolate each of six Candida species was inoculated into blood culture bottles (final concentration, 1–5 CFU ml−1) and incubated at 37 °C until automated growth detection.

Results

Candida albicans and Nakaseomyces glabratus (Candida glabrata) were detected earlier in the specific culture bottle, whereas Candida tropicalis was detected earlier in the nonspecific bottle; Candida parapsilosis, Pichia kudriavzevii (Candida krusei), and Meyerozyma guilliermondii (Candida guilliermondii) presented similar TTD in both bottles.

Conclusions

Our study suggests the suitability of using both bottles in clinical laboratories for a faster diagnosis and prompt starting of any treatment.
背景:念珠菌血症的早期诊断对于患者的正确治疗至关重要:目的:测试不同血液培养瓶对念珠菌菌株生长的功效:我们使用 BD BACTEC™ FX 40 自动血液培养系统比较了 BD BACTEC™ Plus Aerobic/F (Aero) 培养瓶和特定的 BD BACTEC™ Mycosis IC/F Lytic (Myco) 培养瓶的性能,以确定念珠菌菌株的平均检测时间 (TTD)。将六种念珠菌中的每一种接种到血液培养瓶中(最终浓度为 1-5CFUml-1),并在 37°C 温度下培养至自动生长检测:结果:白色念珠菌和光滑念珠菌(Nakaseomyces glabratus)在特异性培养瓶中更早被检测到,而热带念珠菌在非特异性培养瓶中更早被检测到;副丝状念珠菌、Pichia kudriavzevii(克鲁塞念珠菌)和Meyerozyma guilliermondii(吉利蒙念珠菌)在两种培养瓶中的TTD相似:我们的研究表明,在临床实验室中使用这两种瓶子可以更快地做出诊断并迅速开始治疗。
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Revista Iberoamericana De Micologia
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