Taphonomy of experimental burials in Taphos-m: The role of fungi

IF 1.5 4区生物学 Q4 MYCOLOGY Revista Iberoamericana De Micologia Pub Date : 2021-07-01 DOI:10.1016/j.riam.2021.02.003

Aida Gutiérrez , Laia Guàrdia , Dominika Nociarová , Assumpció Malgosa , Núria Armentano

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引用次数: 1

Abstract

Background

The fungi present in the decaying remains enable a better understanding of the processes of decomposition after death. There are not many studies about fungi on decaying bodies and it is not known which fungal sampling methods are effective.

Aims

The main objective of this study was to find the best method for sampling fungi in carcasses, prove the effectiveness of this method and identify the fungal colonies in animal carcasses from experimental burials.

Methods

Samples from 13 carcasses of Sus scrofa domestica, from the experimental project Taphos-m, were taken with different materials: spatula, sterile swabs and RODAC contact plates.

Results

RODAC contact plates with the RBA culture medium showed higher proliferation of fungal colonies. Thirty genera of fungi were isolated from different substrates (bone, tissue, lime). Most of the fungi genera or groups identified have been described before in the literature, but the substrates they came from were different in some cases.

Conclusions

Sampling with RODAC contact plates was found to be the most effective method, as it provides a nutritional culture medium that may allow growth since the moment of sampling. Fungi colonies grew better in RBA culture medium because bacterial growth is inhibited. Most of the observed fungi are related to the environment but some others have been found related to decomposing bodies for the first time.

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Taphos-m实验埋藏的埋藏学:真菌的作用

腐尸中存在的真菌使我们能够更好地理解人死后的分解过程。关于腐尸真菌的研究并不多，也不知道哪种真菌取样方法是有效的。目的探讨动物尸体真菌取样的最佳方法，验证该方法的有效性，并对实验掩埋动物尸体真菌菌落进行鉴定。方法采用刮刀、无菌拭子、RODAC接触片等不同材料采集13只家蝇标本。结果rodac接触板与RBA培养基具有较高的真菌菌落增殖率。从不同基质(骨、组织、石灰)中分离出30属真菌。大多数已鉴定的真菌属或类群已经在文献中描述过，但在某些情况下，它们来自不同的底物。结论RODAC接触板取样是最有效的方法，因为它提供了一种营养培养基，从取样的那一刻起就可以生长。真菌菌落在RBA培养基中生长较好，因为细菌生长受到抑制。大多数观察到的真菌与环境有关，但也有一些是首次发现与腐烂的尸体有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.