Imaging lipids in biological samples with surface-assisted laser desorption/ionization mass spectrometry: A concise review of the last decade

IF 14 1区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Progress in lipid research Pub Date : 2021-07-01 DOI:10.1016/j.plipres.2021.101114

Wendy H. Müller, Edwin De Pauw, Johann Far, Cedric Malherbe, Gauthier Eppe

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引用次数: 14

Abstract

Knowing the spatial location of the lipid species present in biological samples is of paramount importance for the elucidation of pathological and physiological processes. In this context, mass spectrometry imaging (MSI) has emerged as a powerful technology allowing the visualization of the spatial distributions of biomolecules, including lipids, in complex biological samples. Among the different ionization methods available, the emerging surface-assisted laser desorption/ionization (SALDI) MSI offers unique capabilities for the study of lipids. This review describes the specific advantages of SALDI-MSI for lipid analysis, including the ability to perform analyses in both ionization modes with the same nanosubstrate, the detection of lipids characterized by low ionization efficiency in MALDI-MS, and the possibilities of surface modification to improve the detection of lipids. The complementarity of SALDI and MALDI-MSI is also discussed. Finally, this review presents data processing strategies applied in SALDI-MSI of lipids, as well as examples of applications of SALDI-MSI in biomedical lipidomics.

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表面辅助激光解吸/电离质谱成像生物样品中的脂质:近十年的简要回顾

了解生物样品中存在的脂质种类的空间位置对于阐明病理和生理过程至关重要。在这种背景下，质谱成像(MSI)已经成为一种强大的技术，允许在复杂的生物样品中可视化生物分子的空间分布，包括脂质。在可用的不同电离方法中，新兴的表面辅助激光解吸/电离(SALDI) MSI为脂质研究提供了独特的能力。这篇综述描述了SALDI-MSI在脂质分析方面的特殊优势，包括在两种电离模式下使用相同的纳米底物进行分析的能力，在MALDI-MS中检测以低电离效率为特征的脂质，以及表面修饰以提高脂质检测的可能性。并讨论了SALDI和MALDI-MSI的互补性。最后，本文综述了SALDI-MSI在脂质数据处理中的应用策略，以及SALDI-MSI在生物医学脂质组学中的应用实例。

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来源期刊

Progress in lipid research 生物-生化与分子生物学

CiteScore

24.50

自引率

2.20%

发文量

审稿时长

14.6 weeks

期刊介绍： The significance of lipids as a fundamental category of biological compounds has been widely acknowledged. The utilization of our understanding in the fields of biochemistry, chemistry, and physiology of lipids has continued to grow in biotechnology, the fats and oils industry, and medicine. Moreover, new aspects such as lipid biophysics, particularly related to membranes and lipoproteins, as well as basic research and applications of liposomes, have emerged. To keep up with these advancements, there is a need for a journal that can evaluate recent progress in specific areas and provide a historical perspective on current research. Progress in Lipid Research serves this purpose.