Magnetic dual-responsive semi-IPN nanogels based on chitosan/PNVCL and study on BSA release behavior.

IF 4.4 3区医学 Q2 ENGINEERING, BIOMEDICAL Progress in Biomaterials Pub Date : 2021-09-01 Epub Date: 2021-08-09 DOI:10.1007/s40204-021-00161-8

Hamed Mohammad Gholiha, Morteza Ehsani, Ardeshir Saeidi, Azam Ghadami, Najmeh Alizadeh

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引用次数: 3

Abstract

Magnetic thermoresponsive nanogels present a promising new approach for targeted drug delivery. In the present study, bovine serum albumin (BSA) loaded thermo-responsive magnetic semi-IPN nanogels (MTRSI-NGs) were developed. At first poly(N-vinyl caprolactam) (PNVCL) was synthesized by free radical polymerization and then MTRSI-NGs were prepared by crosslinking chitosan in presence of chitosan and Fe₃O₄. The formation of MTRSI-NGs has been confirmed by FTIR, and the average molecular weight of PNVCL was determined by GPC analysis. Rheological and turbidimetry analysis were used to determine lower critical solution temperature (LCST) of PNVCL and magnetic thermo-responsive nanogels (MTRSI-NGs) around 32 and 37 °C, respectively. FE-SEM analysis showed particle size at less than 20 nm in the dried state. Dynamic light scattering determined particle size at about 30 nm in a swelling state. The analysis of release behavior showed that the BSA release ratio at 40 °C was faster than 25 °C. The pH release behavior was evaluated at pH 5.5 and 7.4 and showed that the drug release rate at pH 5.5 was more rapid than pH 7.4. The results show MTRSI-NGs are applicable to protein targeted delivery by thermosensitive targeted drug delivery systems.

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壳聚糖/PNVCL磁性双响应半ipn纳米凝胶及其BSA释放行为研究。

磁性热响应纳米凝胶是一种很有前途的靶向药物递送新方法。在本研究中，研制了牛血清白蛋白(BSA)负载热响应磁性半ipn纳米凝胶(MTRSI-NGs)。首先采用自由基聚合法制备了聚n -乙烯基己内酰胺(PNVCL)，然后在壳聚糖和Fe3O4存在下用壳聚糖交联制备了MTRSI-NGs。FTIR证实了MTRSI-NGs的形成，GPC分析确定了PNVCL的平均分子量。采用流变学和浊度法分别测定了PNVCL和磁性热响应纳米凝胶(MTRSI-NGs)在32℃和37℃左右的较低临界溶液温度(LCST)。FE-SEM分析表明，干燥状态下的颗粒尺寸小于20 nm。动态光散射测定了膨胀状态下约30 nm处的颗粒尺寸。释放行为分析表明，在40°C时，BSA的释放比25°C时更快。在pH为5.5和7.4时对药物的释放行为进行了评价，结果表明，pH为5.5时药物的释放速度比pH为7.4时更快。结果表明，MTRSI-NGs可用于热敏靶向药物递送系统的蛋白质靶向递送。

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来源期刊

Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

9.60

自引率

4.10%

发文量

期刊介绍： Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.