Inhibition of hypoxia-inducible factor-1 by salidroside in an in vitro model of choroidal neovascularization.

IF 1.6 4区医学 Q3 OPHTHALMOLOGY Cutaneous and Ocular Toxicology Pub Date : 2022-09-01 Epub Date: 2022-07-15 DOI:10.1080/15569527.2021.1973023

Haitao Yang, Qingwu Yang, Linfei Zheng

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引用次数: 1

Abstract

Purpose: As a characteristic of age-related macular degeneration (AMD), choroidal neovascularization (CNV) causes severe vision loss. The current treatment has limited efficacy. This study was to investigate effects of Salidroside against CNV and explore its underlying mechanisms.

Methods: RF/6A cells were treated with 200 mM cobalt chloride (CoCl₂) for 6 hr to mimic hypoxic condition. Cells were then treated with Salidroside at 10, 30, and 100 µM for 24 hr. Cells treated with DMSO were used as negative control. The cell proliferation was assessed using 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium-bromid assay. The tube formation was investigated on Matrigel. The cell migration was measured by a Transwell assay. RT-qPCR was used to detect the gene expression. Immuohistochemistry and western blot were used to detect the expression of proteins.

Results: Salidroside significantly inhibited the cell migration and tube formation activity of RF/6A cells under hypoxia. Moreover, Salidroside reduced the expression levels of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 (HIF-1) in RF/6A cells.

Conclusions: Our data suggested that Salidroside could be a potential novel therapeutic agent against CNV.

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红景天苷对脉络膜新生血管体外模型中缺氧诱导因子-1的抑制作用。

目的:作为老年性黄斑变性(AMD)的一个特征，脉络膜新生血管(CNV)会导致严重的视力丧失。目前的治疗效果有限。本研究旨在探讨红景天苷抗CNV的作用及其机制。方法:用200 mM氯化钴(CoCl2)处理RF/6A细胞6小时，模拟缺氧状态。然后用红景天苷在10、30和100µM条件下处理细胞24小时。用DMSO处理的细胞作为阴性对照。采用3-(4,5-二甲基噻唑)-2,5-二苯四唑-溴化法测定细胞增殖情况。在Matrigel上对管的形成进行了研究。用Transwell法测定细胞迁移量。RT-qPCR检测基因表达。免疫组织化学和western blot检测蛋白表达。结果:红景天苷显著抑制缺氧条件下RF/6A细胞的迁移和成管活性。红景天苷降低了RF/6A细胞中血管内皮生长因子(VEGF)和缺氧诱导因子-1 (HIF-1)的表达水平。结论:我们的数据提示红景天苷可能是一种潜在的新型治疗CNV的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cutaneous and Ocular Toxicology 医学-毒理学

CiteScore

3.30

自引率

6.20%

发文量

审稿时长

1 months

期刊介绍： Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.