Antibacterial Activity and Mechanism of ZnO/Cu²⁺-Chitosan/Montmorillonite.

IF 1.3 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Wuhan University of Technology-Materials Science Edition Pub Date : 2021-01-01 Epub Date: 2021-08-29 DOI:10.1007/s11595-021-2438-2

Xiaoxia Ma, Lingjuan Gao, Yulong Ma, Xiaoguang Zhang

{"title":"Antibacterial Activity and Mechanism of ZnO/Cu2+-Chitosan/Montmorillonite.","authors":"Xiaoxia Ma, Lingjuan Gao, Yulong Ma, Xiaoguang Zhang","doi":"10.1007/s11595-021-2438-2","DOIUrl":null,"url":null,"abstract":"A new composite antibacterial material ZnO/Cu2+-Chitosan/Montmorillonite (ZCCM) was prepared with montmorillonite as carrier, Zn(Ac)2•2H2O, Cu(NO3)2•3H2O and chitosan as raw materials. ZCCM was characterized by X-ray diffraction, nitrogen physical adsorption, scanning electron microscopy and energy dispersion spectrometry. The antibacterial activity of ZCCM against Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus was evaluated by minimal inhibitory concentration, minimum bactericidal concentration and the influence of growth curves. ZCCM displays excellent antibacterial activity which is higher than ZnO-Montmorillonite, Cu2+-Montmorillonite and ZnO/Cu2+-Montmorillonite. In addition, the antibacterial mechanism of ZCCM was investigated by analyzing bacterial morphology, integrity of cell membrane, lipid peroxidation and the effect of histidine on antibacterial activity of materials. It is found that cell morphologies of bacteria are damaged and bacterial cells are shrunken. With the increase of cell membrane permeability, the intracellular dissolved matters leak continuously. What's more, the reactive oxygen species are generated and biomacromolecules are oxidized.","PeriodicalId":49949,"journal":{"name":"Journal of Wuhan University of Technology-Materials Science Edition","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403250/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Wuhan University of Technology-Materials Science Edition","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1007/s11595-021-2438-2","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/8/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 2

Abstract

A new composite antibacterial material ZnO/Cu²⁺-Chitosan/Montmorillonite (ZCCM) was prepared with montmorillonite as carrier, Zn(Ac)₂•2H₂O, Cu(NO₃)₂•3H₂O and chitosan as raw materials. ZCCM was characterized by X-ray diffraction, nitrogen physical adsorption, scanning electron microscopy and energy dispersion spectrometry. The antibacterial activity of ZCCM against Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus was evaluated by minimal inhibitory concentration, minimum bactericidal concentration and the influence of growth curves. ZCCM displays excellent antibacterial activity which is higher than ZnO-Montmorillonite, Cu²⁺-Montmorillonite and ZnO/Cu²⁺-Montmorillonite. In addition, the antibacterial mechanism of ZCCM was investigated by analyzing bacterial morphology, integrity of cell membrane, lipid peroxidation and the effect of histidine on antibacterial activity of materials. It is found that cell morphologies of bacteria are damaged and bacterial cells are shrunken. With the increase of cell membrane permeability, the intracellular dissolved matters leak continuously. What's more, the reactive oxygen species are generated and biomacromolecules are oxidized.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

ZnO/Cu2+-壳聚糖/蒙脱土的抗菌活性及其机理

以蒙脱土为载体，Zn(Ac)2•2H2O、Cu(NO3)2•3H2O和壳聚糖为原料，制备了ZnO/Cu2+-壳聚糖/蒙脱土(ZCCM)复合抗菌材料。采用x射线衍射、氮物理吸附、扫描电镜和能谱分析对ZCCM进行了表征。通过最小抑菌浓度、最小杀菌浓度和生长曲线的影响来评价ZCCM对大肠杆菌、鼠伤寒沙门菌和金黄色葡萄球菌的抑菌活性。ZCCM具有优异的抑菌活性，其抑菌活性高于ZnO-蒙脱土、Cu2+-蒙脱土和ZnO/Cu2+-蒙脱土。此外，通过分析细菌形态、细胞膜完整性、脂质过氧化和组氨酸对材料抗菌活性的影响，探讨了ZCCM的抗菌机制。发现细菌的细胞形态被破坏，细菌细胞萎缩。随着细胞膜通透性的增加，细胞内溶出物不断渗漏。更重要的是，活性氧的生成和生物大分子的氧化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Wuhan University of Technology-Materials Science Edition 工程技术-材料科学：综合

CiteScore

2.10

自引率

12.50%

发文量

136

审稿时长

5.5 months

期刊介绍： The Journal publishes reviews, full-length papers, and short communications presenting original research in materials science and engineering, focusing on functional and structural materials, advanced technology for materials synthesis and processing, nanomaterials, composites, computational material science, etc. All papers published are subjected to peer review, and are edited to conform to related international standards.