Osteogenic Induction of Low-dose Ipriflavone on Bone Marrow Mesenchymal Stem Cells Extracted from Osteoporosis Rats.

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2021-09-07 DOI:10.3290/j.cjdr.b1965039

Xiao Wang, Ao Zheng, Xian Zhen Xin, Ling Jie Peng, Jie Wang, Ling Yan Cao, Xin Quan Jiang

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引用次数: 2

Abstract

Objective: To investigate the effects of ipriflavone (IPF), a synthetic isoflavone plant oestrogen with a structure similar to that of oestradiol, on the osteogenic differentiation of bone mesenchymal stem cells (BMSCs).

Methods: BMSCs were derived from ovariectomised rats (rBMSCs-OVX) and then induced with or without IPF. Cell cytotoxicity, mineralisation in vitro and osteoblast-specific gene expression of BMSCs were studied.

Results: IPF at a concentration of 10-8, 10-7 and 10-6 mol/l exhibited no cytotoxic effect on the proliferation of BMSCs but increased alkaline phosphatase activity and osteoblast-specific gene expression.

Conclusion: IPF enhances osteogenic differentiation of rBMSCs-OVX partly in vitro, thus its use offers a potential strategy for the treatment of osteoporosis.

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低剂量依普利黄酮对骨质疏松大鼠骨髓间充质干细胞成骨的诱导作用。

目的:研究异丙黄酮(ipriflavone, IPF)对骨间充质干细胞(BMSCs)成骨分化的影响。异丙黄酮是一种与雌二醇结构相似的合成异黄酮植物雌激素。方法:从去卵巢大鼠(rBMSCs-OVX)中提取骨髓间充质干细胞，用或不加IPF诱导。研究了骨髓间充质干细胞的细胞毒性、体外矿化和成骨特异性基因表达。结果:10-8、10-7和10-6 mol/l浓度的IPF对骨髓间充质干细胞增殖无细胞毒作用，但增加碱性磷酸酶活性和成骨细胞特异性基因表达。结论:IPF在体外部分促进rBMSCs-OVX成骨分化，为治疗骨质疏松症提供了一种潜在的策略。

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The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)

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