The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)最新文献

Objective: To assess the impact of hyperbaric oxygen (HBO) therapy on bone healing in dia-betic patients and explore potential mechanisms in an animal experiment.

Methods: Four non-diabetic patients undergoing maxillary sinus lateral augmentation were assigned to either standard care (control group) or HBO treatment for 5, 6 or 9 weeks. Bone core samples were collected for immunochemistry during the preparation for implant insertion. Additionally, 12 db/db diabetic mice and 12 control mice with tibia defects were subjected to HBO treatment. After 7 days, the bone repair process was assessed using microcomputed tom-ography (microCT), histology, immunohistochemistry and Western blot analyses.

Results: HBO improved bone formation in the implant areas of patients treated for 5, 6 and 9 weeks, increasing local vascular endothelial growth factor, bone morphogenetic protein-2 and osteocalcin expression. Diabetic mice exhibited impaired bone healing, but HBO-treated groups (diabetic or non-diabetic) showed enhanced new bone formation. Increased osteocalcin and runt-related transcription factor 2 and decreased tartrate-resistant acid phosphatase expression were detected. The nuclear factor erythroid-derived 2-related factor 2 (Nrf2) pathway and its downstream proteins catalase and heme oxygenase-1 were upregulated after HBO.

Conclusion: HBO enhances bone healing in both patients and diabetic mice, and is potentially mediated through the Nrf2 pathway.

Objective: To compare the effects on the oral microbiome after rinsing with hydrogen peroxide (H2O2), chlorhexidine and essential oil mouthrinses and identify formulations that suppress pathogenic bacteria while preserving beneficial species and maintaining oral microbial bal-ance.

Methods: Twelve healthy volunteers were randomly assigned to three groups: H2O2, chlorhexi-dine and essential oil mouthrinse. Saliva samples were collected at three time points: before and 5 minutes and 1 hour after rinsing with mouthrinse. Microbiome composition was analysed using 16S rRNA gene sequencing.

Results: Alpha and beta diversity showed no statistically significant differences among time points. The genus-level microbiome composition remained relatively stable in the H2O2 and essential oil groups but changed significantly in the chlorhexidine group. In the H2O2 group, Neisseria decreased significantly, while Actinomyces increased. In the chlorhexidine group, Porphyromonas, Veillonella, Streptococcus, Neisseria and Gemella decreased significantly. In the essential oil group, Leptotrichia decreased, and Haemophilus increased significantly.

Conclusion: Essential oil mouthrinse and chlorhexidine exhibit stronger bacteriostatic effects against oral pathogens than H2O2. However, chlorhexidine may disrupt microbial equilibrium, whereas essential oil mouthrinse more effectively preserves a stable oral microbiome. Thus, es-sential oil mouthrinse could serve as a viable alternative to chlorhexidine for oral microbiome management, though its long-term efficacy requires further investigation.

Objective: To develop an efficient and accurate method for the precise quantification of peri-implant marginal bone loss, aiming to overcome the limitations of conventional assessments that rely heavily on expert subjective judgment and to enhance the monitoring of marginal bone level stability and facilitate the early detection of clinical risk factors.

Methods: This research proposes an efficient automatic quantification method for marginal bone loss based on the YOLOv8-pose model. By detecting and localising keypoints associated with peri-implant bone loss, this method enables automated identification of marginal bone loss characteristics. Model performance is evaluated using the marginal bone loss rate to assess its effectiveness in predicting the severity of resorption on both sides of the implant.

Results: The precision of keypoint detection for marginal bone loss features exceeds 98%, and the mean radial error of six keypoints in most images is less than 10 pixels, with the minimum error reaching only 2.89 pixels. In predicting marginal bone loss severity on both implant sides, the model achieves accuracies of 0.906 and 0.844, respectively, demonstrating robust predictive capability and application potential.

Conclusion: The YOLOv8-pose-based method effectively addresses the inefficiency and subjec-tivity of traditional assessments by enhancing the automation and objectivity of peri-implant marginal bone loss detection. It provides valuable technical support for accurate evaluation and clinical decision making in peri-implant marginal bone loss.

Mechanotransduction is the biological process by which physical forces are converted into biochemical signals, playing a central role in alveolar bone remodelling. This remodelling is influenced by external stimuli such as mechanical stress, bone loss, and medication-related osteonecrosis. While most in vivo studies have focused on mechanotransductive changes within the periodontal ligament (PDL), it is increasingly recognised that the alveolar bone itself plays a broader and more complex role in this dynamic process. This narrative review presents a quali-tative synthesis of literature sourced from PubMed, Scopus and Web of Science, emphasising the cellular and molecular pathways involved in mechanotransduction within alveolar bone. A deeper understanding of these mechanisms is essential for advancing applications in orthodon-tic tooth movement, dental implant osseointegration, and musculoskeletal tissue engineering. Moreover, insights into the biomechanical regulation of bone may facilitate the development of improved biomaterials and regenerative strategies in clinical dentistry and orthopaedics.

Objective: To explore the association of PAX3 gene variants and non-syndromic orofacial cleft lip/palate (NSCL/P) among the Han Chinese population in Western China.

Methods: A total of 1,626 NSCL/P patients, 886 non-syndromic cleft palate only (NSCPO) pa-tients and 2,255 controls were recruited for association analysis. A total of 8 NSCPO patients, 7 non-syndromic cleft lip only (NSCLO) patients, and 3 normal controls were recruited for RNA sequencing. Blood samples were collected, genotype data for single nucleotide polymorphisms (SNPs) on the PAX3 gene were obtained and multi-level association analyses were conducted to explore their relationship with NSCL/P, including functional predictions of disease-related SNPs. Additionally, RNA sequencing was performed to investigate differences in PAX3 gene expression between patients with NSCL/P and controls. The study was conducted in PLINK analysis.

Results: rs2033806 was significantly associated with NSCL/P, whereas rs1430651 was mar-ginally associated with NSCL/P. None were significantly associated with NSCPO. In addition, rs2033806 was also associated with the subtypes NSCLP and NSCLO.

Conclusion: rs2033806 at PAX3 showed different frequency distributions between non-syn-dromic oral cleft (NSOC) patients and controls, providing new insights into the aetiologies of NSOC.

Photodynamic therapy (PDT) utilising 5-aminolevulinic acid (5-ALA) has emerged as a signifi-cant non-invasive treatment for oral potentially malignant disorders (OPMDs). PDT involves the use of a photosensitiser (5-ALA), light and oxygen to selectively target and destroy diseased tissues via photochemical reactions. This therapy has shown promising results in treating vari-ous OPMDs, such as oral leucoplakia, oral erythroplakia and oral lichen planus. This consensus document outlines the clinical application of 5-ALA-PDT, providing a comprehensive guide for oral mucosal specialists and stomatology departments. The consensus includes detailed recommendations on irradiation dose, irradiation time, light source selection, pre-treatment preparation, clinical diagnosis, treatment protocols, efficacy evaluation and management of post-treatment adverse events. The goal is to standardise 5-ALA-PDT procedures to improve clinical outcomes and provide a scientific basis for the treatment of OPMDs. By offering guid-ance on patient selection, lesion assessment and therapeutic strategies, this document aims to address current clinical challenges and enhance the effectiveness of PDT in managing oral mucosal diseases.

Objective: To compare the marginal fit and mechanical performance of three-unit implant-supported fixed dental prostheses (ISFDPs) fabricated using fully digital and conventional workflows.

Methods: Three-unit ISFDP specimens in this study simulated the restoration of three consecu-tively missing teeth in the mandibular posterior region. Twenty-four specimens were divided into three groups: the zirconia fully digital (ZFD) group received zirconia ISFDPs fabricated using a fully digital workflow; the polyetherketoneketone fully digital (PFD) group received polyetherketoneketone (PEKK) ISFDPs fabricated using a fully digital workflow; and the zir-conia control (ZC) group received zirconia ISFDPs fabricated using a conventional workflow. Marginal gaps between restorations and abutments during passive and active fit were meas-ured, and initial screw loosening torque values of restorations were recorded. After undergoing 1,200,000 cycles of vertical loading, the restorations were reassessed for marginal gaps and loosening torque values.

Results: Marginal fit under passive and active fit in the ZFD group showed no significant dif-ference in relation to the ZC group. Marginal gaps in the PFD group (134.39 ± 41.13 µm) under passive fit were significantly larger than in the ZFD group, and decreased significantly under active fit (22.28 ± 10.45 µm). No significant difference in marginal fit was observed before and after cyclic loading in all groups. No significant difference was found in loosening torque change between the ZFD and ZC groups, whereas the change in the PFD group (median -2.13%, Q25 -3.30%/Q75 0.28%) was significantly lower than in the ZC group (median -5.83%, Q25 -8.46%/Q75 -3.75%).

Conclusion: Three-unit ISFDPs fabricated using fully digital and conventional workflows exhibit similar marginal fit and mechanical performance. Additionally, PEKK ISFDPs demonstrate clinically acceptable marginal fit and mechanical performance.

Objective: To evaluate the effects of a novel software developed to assist dental students in mak-ing tooth ground sections and gaining knowledge of dental hard tissues.

Methods: Students were divided into three groups (class-use group, after-class-use group and control group) and taught how to make real sections or allowed to use the aforementioned software to make virtual tooth ground sections on the computer. The present authors then assessed the efficiency of making the first tooth ground sections, the quality of ground sections and performance skills.

Results: The sectioning time for the class-use group was less than that for the after-class-use group and control group (P = 0.027), and the quality of sections for the class-use group was higher (P = 0.036). In the objective test 1 week later, the after-class-use group performed better than the control group (P = 0.019). Users agreed that the software stimulated their interest and improved their learning results.

Conclusion: This software is an efficient tool to help students to make tooth ground sections and study the histology of dental hard tissues in a simple and functional way.

Biomineralisation is a remarkable biological process in which living organisms exert precise control over the nucleation and growth of inorganic crystalline phases, resulting in the formation of hierarchically structured biocomposites that exhibit exceptional mechanical and functional properties. Since damage to bone and teeth directly affect everyday life, various biomimetic mineralised materials have been engineered for use in biomedical applications. While bioinspired materials typically demonstrate superior mechanical properties and biological functions, significant disparities remain between biomimetic constructs and their natural counterparts, especially concerning mechanical performance and multiscale structural organisation. This review initially describes the dynamic reciprocity between type I collagen fibrils, amorphous calcium phosphate phases and multifunctional non-collagenous protein within mineralisation microenvironments. Furthermore, it evaluates recent progress in advanced biomaterials based on biomimetic mineralisation strategies and seeks to spark innovative and promising solutions for investigators exploring biomineralisation principles in regenerative medicine and hard tissue reconstruction. Existing problems and future directions are discussed.

Objective: To compare the prevalence and morphological characteristics of canalis sinuosus (CS) between unilateral cleft lip and palate (UCLP), bilateral cleft lip and palate (BCLP) and control groups.

Methods: The sample consisted of 238 CBCT images (476 sides) from 98 UCLP subjects (196 sides), 36 BCLP subjects (72 sides) and 104 healthy controls (208 sides). Recorded parameters included prevalence of CS, diameter, location of the teeth and adjacent structures. Afterwards, the recorded parameters were compared between the UCLP, BCLP and control groups.

Results: The prevalence of CS in the control, UCLP and BCLP groups showed significant differences. The BCLP group revealed a significantly lower prevalence of CS than the UCLP and control groups. There was a considerable increase in CS diameter in the CLP groups compared with the control group. The terminal location of CS was in the canine region for the CLP groups and in the lateral incisor region for the control group. CLP had a significant impact on the location of the end of the CS. CEJB (cementoenamel junction buccal) and CEJL (cementoenamel junction lingual) measurements showed significant differences between the CLP cases and control groups.

Conclusion: Different characteristics was revealed between the control, UCLP and BCLP groups. Assessment of CS in patients with CLP with CBCT images is crucial before performing surgical procedures.